[Objective] To investigate the effects of traditional Chinese medicine compound preparation on serum biochemical indexes and egg nutritional quality of laying hens. [Method] A total of 1 200 chickens at the age of 44 weeks were selected and randomly divided into five groups. After administration with different dosages of astragalus polysaccharides and berberine for 9 weeks,serum biochemical indexes were determined and nutrient contents of egg were detected. [Result] The contents of total protein,globulin,high-density lipoprotein cholesterol(HDL-C) and alkaline phosphatase(ALP) in all treatment groups were significantly(P < 0.05 or 0.01) higher than those in the control group. The cholesterol contents of all treatment groups were significantly(P < 0.05 or 0.01) lower than those of the control group. [Conclusion] The optimal concentrations of astragalus polysaccharides and berberine in basic diets are 200 and 30 mg/kg,respectively. [Objective] To investigate the effects of traditional Chinese medicine compound preparation on serum biochemical indexes and egg nutritional quality of laying hens. [Method] A total of 1 200 chickens at the age of 44 weeks were selected and optionally divided into five groups. After administration with different dosages of astragalus polysaccharides and berberine for 9 weeks, serum biochemical indexes were determined and nutrient contents of egg were detected. [Result] The contents of total protein, globulin, high-density lipoprotein cholesterol (HDL-C) and alkaline phosphatase (ALP) in all treatment groups were significantly (P <0.05 or 0.01) higher than those in the control group. The cholesterol contents of all treatment groups were significantly (P <0.05 or 0.01) lower than those of the control group. ] The optimal concentrations of astragalus polysaccharides and berberine in basic diets are 200 and 30 mg / kg, respectively.