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利用大鼠胚胎肢芽细胞进行原代培养 ,培养物经不同强度的稳恒磁场作用 ,研究稳恒磁场对增殖和分化的影响 ,并利用图象分析细胞形态的变化。结果显示强度大于 40mT的稳恒磁场能明显抑制肢芽细胞增殖和分化 ,集落形成率明显减少 ,并显示剂量效应关系。拟合细胞分化和增殖抑制曲线 ,计算得大鼠胚胎肢芽细胞半数分化抑制强度 (ICD5 0 )为 5 2mT ,半数存活抑制强度 (ICV5 0 )为 40mT。表明抑制细胞分化、增殖可能是稳恒磁场致发育危害的重要作用
The rat embryonic limb bud cells were used for primary culture, and the culture was subjected to steady magnetic field with different intensities to study the effect of the steady magnetic field on proliferation and differentiation. The morphological changes of the cells were analyzed by using the image. The results showed that the steady magnetic field intensity greater than 40mT can significantly inhibit the proliferation and differentiation of limb bud cells, colony formation rate was significantly reduced, and showed dose-response relationship. The inhibition curve of cell differentiation and proliferation was fitted. The IC50 of rat embryonic limb bud cells was 5 2mT and the IC50 of IC50 was 40mT. Indicating that inhibition of cell differentiation and proliferation may be an important role in the development of stable magnetic fields