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为了获得棉花纤维特异启动子,克隆了棉花纤维细胞中表达基因GhSCFP的5′端上游序列,将其分别与GUS和GFP报告基因融合.在转基因烟草中,仅在种皮的最外层细胞中检测到GUS和GFP活性;在转基因陆地棉中,根、叶片、幼茎、花冠、花药和柱头中均未观察到GUS信号,而在开花当天胚珠表面突起的纤维细胞中有明显的GUS活性,直到开花后36d,纤维上仍可观察到GUS信号.本研究结果显示,SCFP启动子具有明显的棉花纤维表达特异性和植物种皮特异性,而且表达强度高.该启动子在棉花纤维发育相关基因的功能研究和棉花基因工程改良中将有重要的应用价值.
In order to obtain a cotton fiber specific promoter, the 5 ’upstream sequence of the GhSCFP expression gene in cotton fiber cells was cloned and fused with GUS and GFP reporter genes, respectively, in transgenic tobacco only in the outermost cells of the seed coat GUS and GFP activities were detected. No GUS signal was observed in roots, leaves, young stems, corolla, anther and stigma in transgenic G. hirsutum, but significant GUS activity was observed in the fiber cells protruding on the ovule on the day of flowering, GUS signal could still be observed on the fiber 36 days after flowering.The results of this study showed that the SCFP promoter has obvious cotton fiber expression specificity and plant seed coat specificity and high expression intensity.This promoter plays an important role in cotton fiber development related gene Function research and cotton genetic engineering will have important application value.