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Abstract： Flower morphology is a key trait for sub-division and inter-species definition of genus Oreocharis. O. rhytidophyllaC. Y. Wu ex H. W. Li was last collected in 1956 and published in 1983， and the species has no relevant collection records for the next 60 years. Due to the lack of flower morphology， there are doubts in Flora of Reipublicae Popularis Sinicae， Plants of Gesneriaceae in Chinaand Flora of China. After years of follow-up investigation， Oreocharis rhytidophyllaC. Y. Wu ex H. W. Li was rediscovered in 2017 in its type locality. Due to the availability of the flowers during the rediscovery， we confirmed that the species should be considered as a natural species instead of doubtful one. The floral morphology of O. rhytidophyllaC. Y. Wu ex H. W. Li was supplemented based on the recent collections and photos. Its rediscovery provides an opportunity to explore its systematic position in the context of phylogeny in the future.
Key words： supplementary description， flower morphology， Oreocharis，Wuliangshan National Nature Reserve
CLC number： Q949
Document code： A
Article ID： 1000-3142（2019）05-0569-05
摘 要： 花部形態是马铃苣苔属属下划分和种间界定的关键性状，缺乏花器官的描述直接导致了一些存疑物种的存在。网叶马铃苣苔（Oreocharis rhytidophylla C. Y. Wu ex H. W. Li）自1956年最后被采集到并于1983年发表，由于没有花的特征，在《中国植物志》《中国苦苣苔科植物》和Flora of China均存疑，但该种在随后的60余年间再无相关的采集记录。作者经过多年的跟踪调查，于2017年在其模式产地重新发现并采集了带花的凭证标本。该文作者根据已经采集到的具花标本，确定这个种是个自然种，并基于新收集到的材料，补充描述了花的形态特征。网叶马铃苣苔的重新发现，为探索其系统位置提供了重要的依据。
关键词： 补充描述， 花形态， 网叶马铃苣苔属， 无量山国家级自然保护区
1 Introduction
The classification systems for OreocharisBenth. were mainly based on flower morphological characters. The flower morphplogical characters of the genus are remarkably diverse and include the symmetry of corolla， the color of corolla， the shape of corolla， the number of stamens and staminodes （Wang et al.， 1990， 1998; Li & Wang， 2004）. In the genus， it is very common that species share similar flowers but totally different leaves or share similar leaves but completely different flowers （Wang et al.， 1990， 1998; Li & Wang， 2004）. For example， Oreocharis parvifloraLei Cai & Z. K. Wu is the most closely related toO. henryanaOliv. which shares flower morphology， but the leaves are completely different （Cai et al.， 2017）. O. purpurataB. Pan， M. Q. Han & Yan Liu is similar toO. pinnatilobata（K. Y. Pan） Mich. Mller & A. Weber in leaf， but different in flower morphology （Han et al.， 2017）. 2 Materials and Methods
Oreocharis rhytidophyllaC. Y. Wu ex H. W. Li had been doubtful in taxonomic treatment until the availability of its flowers in the field. The species was firstly described based on the type specimens without flowers and obviously diagnostic by the reticulate nerves on both sides of the rugose leaves （Li， 1983）. Subsequently， it was excluded in the Chinese list of the genus Oreocharisin the publications and mentioned with a brief note of the above gap of flower morphology （Pan， 1987; Wang et al.， 1990， 1998; Li & Wang， 2004）. We had been to the type locality many times and contacted the staff of Wuliangshan National Nature Reserve in order to find it in the field since 2008， but we did not find until 2017， we collected the specimens with flowers in our survey to the type locality （Jingdong County， Yunnan Province， China）. In the sight of the conservation， the species is very rare for its rediscovery after the last discovery in 1956 indicated by the specimen （P. Y. Qiu 53376） （Li， 1983）. Here， we supplementarily describe its floral characters and suggest to confirm its taxonomic position as a natural species in the expanded genus.
Oreocharis rhytidophylla C. Y. Wu ex H. W. Li in Bull. Bot. Res. 3（2）： 9， photo 5. 1983; K. Y. Pan in Acta Phytotax. Sin. 25（4）： 290. 1987.
Type CHINA. Yunnan： Jingdong County， Wuliangshan Mt.， Modaohe Village， the foot of Jiaoding mountain， 2 November 1956， P. Y. Qiu 53376 （ holotype， KUN0484429！; isotype， KUN0548908！， PE00030857-60！ ）.
3 Result and Analysis
3.1 Supplementary description of flowers
Inflorescences axillary， cymes 2-4， 2-4-branched， 4-16-flowered; peduncle densely long brown lanate， up to 14 cm; bracts 3， verticillate， 12-15 × 2.5-3 mm， aba-xially brown lanate， adaxial glabrous. Calyx 5-parted near to base， lobes equal， oblong-lanceolate， 10-12 × 1.1-1.2 mm， margin integrate， apex obtuse， adaxial glabrous， abaxial brown lanate. Corolla yellow， 3.5-3.7 cm long， glabrous; corolla tube cylindric， 2.7-3 cm long， 5-6 mm in diam.; adaxial lip 2-lobed， lobes equal， lobes oblong， 5-6 × 3.6-4 mm， apex round or obtuse， abaxial lip 3-lobed， lobes oblong， lobes nearly equal， 6-8 × 3-5 mm， apex round; stamens 4， included， adaxial anthers coherent in pair， abaxial anthers free， adaxial stamens 1.7-2 cm long， adnate to corolla tube 8-9 mm from base， abaxial stamens 1.6-1.8 cm long， adnate to corolla tube 1.4-1.5 cm from base; filaments white， slender， linear， densely glandular-pubescent; anthers oblong， 2-loculed， dehiscing broadwise; staminode 1， clavate， 0.5 mm long， adnate to abaxial side of corolla tube near base. Pistil 2.7-3.0 cm long， glandular pubescent; ovary linear with ridges， 1.7-1.8 cm long， glabrous; style 1-1.2 cm long; stigma 1， disciform， retuse. Disc ring-shaped， yellowish， 2-2.1 mm high， margin slightly undulate with 5 irregularly and shallow lobes. 3.2 Additional specimens examined
CHINA. Yunnan： Jingdong County， Lingjie Town， Modaohe Village， in broad-leaved forests， 24°24′57.17″ N， 100°38′19.26″ E， 2 331 m a. s. l.， in flowers， 30 August 2017， S. W. Guo. B2017-1083 （KUN！）. The same place， 2 200 m a.s.l.， 12 January， 1939， M. K. Li2936 （KUN 0208330！， KUN 075288！， KUN 075289！）.
3.3 Distribution， habitat and phenology
Oreocharis rhytidophylla C. Y. Wu ex H. W. Li（Li， 1983） is only known in South Yunnan and grows in evergreen and deciduous broad-leaved mixed forest， on moist rocks or cliffs at an elevation of ca. 2 331 m. Flowering from August to September and fruiting from September to October.
4 Discussion
Oreocharis rhytidophyllaC. Y. Wu ex H. W. Li is similar to O. benthamia var. reticulata Dunn in netted venation of leaves， but it differs from the latter by its adaxial surface strongly bullate （Pan， 1987; Wang et al.， 1990， 1998; Li & Wang ， 2004）. It is also similar to O. hirsutaBarnett and O. yunnanensisRossini & J. Freitas in the corolla characters， but it differs from the latters by its anthers coherent in pairs and abaxial anthers free （Tan et al.， 2013; Rossini & Freitas， 2014）. It is also similar to O. hekouensis（Y. M. Shui & W. H. Chen） Mich. Miller & A. Weber in the elliptic leaf blade， but it differs from the latter in its obvious reticulate abaxial leaf surface （Chen & Shui， 2006; Mller et al.， 2011）.
Acknowledgement We thank Managing Bureau of Jingdong Wuliangshan National Nature Reserve for supporting our work in the field.
References：
CAI L， HUANG H， DAO ZL， et al.， 2017. Oreocharis parvi-flora， a new species of Gesneriaceae from northwestern Yunnan， China[J]. Phytotaxa， 329（2）： 167-172.
CHEN WH， SHUI YM， 2006.Ancylostemon hekouensis（Gesneriaceae）， a new species from Yunnan， China[J]. Ann Bot Fenn， 43： 448-450.
HAN MQ， PAN B， ZOU LL， et al.， 2017. Oreocharis purpurata， a new species of Gesneriaceae from Hunan， China[J]. Phytotaxa， 328（2）： 183-188.
LI HW， 1983. Notulae De Gesneraceis Yunnanensibus[J]. Bull Bot Res， 3（2）： 1-55.
LI ZY， WANG YZ， 2004. OreocharisBenth. [M]// Plants of Gesneriaceae in China. Zhengzhou： Henan Science & Technology Publish House： 14-47.
MELLER M， MIDDLETON D， NISHII K， et al.， 2011. A new delineation for Oreocharisincorporating an additional ten genera of Chinese Gesneriaceae[J]. Phytotaxa， 23： 1-36.
PAN KY， 1987. Taxonomy of the genus Oreocharis（Gesneria-ceae） [J]. Acta Phytotax Sin， 25： 264-293.
ROSSINI J， FREITAS J， 2014. Oreocharis yunnanensis， a new name for the illegitimate Oreocharis glandulosa（Gesneria-ceae） from China[J]. Phytotaxa， 163 （3）： 180-180.
TAN YH， LI JW， PAN B， et al.， 2013. Oreocharis glandulosa， a new species of Gesneriaceae from southern Yunnan， China[J]. Phytotaxa， 131， 29-34.
WANG WT， PAN KY， LI ZY， 1990. Gesneriaceae [M]// WANG WT. Flora Reipublicae Popularis Sinicae. Beijing： Science Press， 69： 190-203.
WANG WT， PAN KY， LI ZY，et al.， 1998. Gesneriaceae [M]//WU ZY， RAVEN PH. Flora of China. Beijing： Science Press; St. Louis： Missouri Bot Garden， 18： 268-272.
Key words： supplementary description， flower morphology， Oreocharis，Wuliangshan National Nature Reserve
CLC number： Q949
Document code： A
Article ID： 1000-3142（2019）05-0569-05
摘 要： 花部形態是马铃苣苔属属下划分和种间界定的关键性状，缺乏花器官的描述直接导致了一些存疑物种的存在。网叶马铃苣苔（Oreocharis rhytidophylla C. Y. Wu ex H. W. Li）自1956年最后被采集到并于1983年发表，由于没有花的特征，在《中国植物志》《中国苦苣苔科植物》和Flora of China均存疑，但该种在随后的60余年间再无相关的采集记录。作者经过多年的跟踪调查，于2017年在其模式产地重新发现并采集了带花的凭证标本。该文作者根据已经采集到的具花标本，确定这个种是个自然种，并基于新收集到的材料，补充描述了花的形态特征。网叶马铃苣苔的重新发现，为探索其系统位置提供了重要的依据。
关键词： 补充描述， 花形态， 网叶马铃苣苔属， 无量山国家级自然保护区
1 Introduction
The classification systems for OreocharisBenth. were mainly based on flower morphological characters. The flower morphplogical characters of the genus are remarkably diverse and include the symmetry of corolla， the color of corolla， the shape of corolla， the number of stamens and staminodes （Wang et al.， 1990， 1998; Li & Wang， 2004）. In the genus， it is very common that species share similar flowers but totally different leaves or share similar leaves but completely different flowers （Wang et al.， 1990， 1998; Li & Wang， 2004）. For example， Oreocharis parvifloraLei Cai & Z. K. Wu is the most closely related toO. henryanaOliv. which shares flower morphology， but the leaves are completely different （Cai et al.， 2017）. O. purpurataB. Pan， M. Q. Han & Yan Liu is similar toO. pinnatilobata（K. Y. Pan） Mich. Mller & A. Weber in leaf， but different in flower morphology （Han et al.， 2017）. 2 Materials and Methods
Oreocharis rhytidophyllaC. Y. Wu ex H. W. Li had been doubtful in taxonomic treatment until the availability of its flowers in the field. The species was firstly described based on the type specimens without flowers and obviously diagnostic by the reticulate nerves on both sides of the rugose leaves （Li， 1983）. Subsequently， it was excluded in the Chinese list of the genus Oreocharisin the publications and mentioned with a brief note of the above gap of flower morphology （Pan， 1987; Wang et al.， 1990， 1998; Li & Wang， 2004）. We had been to the type locality many times and contacted the staff of Wuliangshan National Nature Reserve in order to find it in the field since 2008， but we did not find until 2017， we collected the specimens with flowers in our survey to the type locality （Jingdong County， Yunnan Province， China）. In the sight of the conservation， the species is very rare for its rediscovery after the last discovery in 1956 indicated by the specimen （P. Y. Qiu 53376） （Li， 1983）. Here， we supplementarily describe its floral characters and suggest to confirm its taxonomic position as a natural species in the expanded genus.
Oreocharis rhytidophylla C. Y. Wu ex H. W. Li in Bull. Bot. Res. 3（2）： 9， photo 5. 1983; K. Y. Pan in Acta Phytotax. Sin. 25（4）： 290. 1987.
Type CHINA. Yunnan： Jingdong County， Wuliangshan Mt.， Modaohe Village， the foot of Jiaoding mountain， 2 November 1956， P. Y. Qiu 53376 （ holotype， KUN0484429！; isotype， KUN0548908！， PE00030857-60！ ）.
3 Result and Analysis
3.1 Supplementary description of flowers
Inflorescences axillary， cymes 2-4， 2-4-branched， 4-16-flowered; peduncle densely long brown lanate， up to 14 cm; bracts 3， verticillate， 12-15 × 2.5-3 mm， aba-xially brown lanate， adaxial glabrous. Calyx 5-parted near to base， lobes equal， oblong-lanceolate， 10-12 × 1.1-1.2 mm， margin integrate， apex obtuse， adaxial glabrous， abaxial brown lanate. Corolla yellow， 3.5-3.7 cm long， glabrous; corolla tube cylindric， 2.7-3 cm long， 5-6 mm in diam.; adaxial lip 2-lobed， lobes equal， lobes oblong， 5-6 × 3.6-4 mm， apex round or obtuse， abaxial lip 3-lobed， lobes oblong， lobes nearly equal， 6-8 × 3-5 mm， apex round; stamens 4， included， adaxial anthers coherent in pair， abaxial anthers free， adaxial stamens 1.7-2 cm long， adnate to corolla tube 8-9 mm from base， abaxial stamens 1.6-1.8 cm long， adnate to corolla tube 1.4-1.5 cm from base; filaments white， slender， linear， densely glandular-pubescent; anthers oblong， 2-loculed， dehiscing broadwise; staminode 1， clavate， 0.5 mm long， adnate to abaxial side of corolla tube near base. Pistil 2.7-3.0 cm long， glandular pubescent; ovary linear with ridges， 1.7-1.8 cm long， glabrous; style 1-1.2 cm long; stigma 1， disciform， retuse. Disc ring-shaped， yellowish， 2-2.1 mm high， margin slightly undulate with 5 irregularly and shallow lobes. 3.2 Additional specimens examined
CHINA. Yunnan： Jingdong County， Lingjie Town， Modaohe Village， in broad-leaved forests， 24°24′57.17″ N， 100°38′19.26″ E， 2 331 m a. s. l.， in flowers， 30 August 2017， S. W. Guo. B2017-1083 （KUN！）. The same place， 2 200 m a.s.l.， 12 January， 1939， M. K. Li2936 （KUN 0208330！， KUN 075288！， KUN 075289！）.
3.3 Distribution， habitat and phenology
Oreocharis rhytidophylla C. Y. Wu ex H. W. Li（Li， 1983） is only known in South Yunnan and grows in evergreen and deciduous broad-leaved mixed forest， on moist rocks or cliffs at an elevation of ca. 2 331 m. Flowering from August to September and fruiting from September to October.
4 Discussion
Oreocharis rhytidophyllaC. Y. Wu ex H. W. Li is similar to O. benthamia var. reticulata Dunn in netted venation of leaves， but it differs from the latter by its adaxial surface strongly bullate （Pan， 1987; Wang et al.， 1990， 1998; Li & Wang ， 2004）. It is also similar to O. hirsutaBarnett and O. yunnanensisRossini & J. Freitas in the corolla characters， but it differs from the latters by its anthers coherent in pairs and abaxial anthers free （Tan et al.， 2013; Rossini & Freitas， 2014）. It is also similar to O. hekouensis（Y. M. Shui & W. H. Chen） Mich. Miller & A. Weber in the elliptic leaf blade， but it differs from the latter in its obvious reticulate abaxial leaf surface （Chen & Shui， 2006; Mller et al.， 2011）.
Acknowledgement We thank Managing Bureau of Jingdong Wuliangshan National Nature Reserve for supporting our work in the field.
References：
CAI L， HUANG H， DAO ZL， et al.， 2017. Oreocharis parvi-flora， a new species of Gesneriaceae from northwestern Yunnan， China[J]. Phytotaxa， 329（2）： 167-172.
CHEN WH， SHUI YM， 2006.Ancylostemon hekouensis（Gesneriaceae）， a new species from Yunnan， China[J]. Ann Bot Fenn， 43： 448-450.
HAN MQ， PAN B， ZOU LL， et al.， 2017. Oreocharis purpurata， a new species of Gesneriaceae from Hunan， China[J]. Phytotaxa， 328（2）： 183-188.
LI HW， 1983. Notulae De Gesneraceis Yunnanensibus[J]. Bull Bot Res， 3（2）： 1-55.
LI ZY， WANG YZ， 2004. OreocharisBenth. [M]// Plants of Gesneriaceae in China. Zhengzhou： Henan Science & Technology Publish House： 14-47.
MELLER M， MIDDLETON D， NISHII K， et al.， 2011. A new delineation for Oreocharisincorporating an additional ten genera of Chinese Gesneriaceae[J]. Phytotaxa， 23： 1-36.
PAN KY， 1987. Taxonomy of the genus Oreocharis（Gesneria-ceae） [J]. Acta Phytotax Sin， 25： 264-293.
ROSSINI J， FREITAS J， 2014. Oreocharis yunnanensis， a new name for the illegitimate Oreocharis glandulosa（Gesneria-ceae） from China[J]. Phytotaxa， 163 （3）： 180-180.
TAN YH， LI JW， PAN B， et al.， 2013. Oreocharis glandulosa， a new species of Gesneriaceae from southern Yunnan， China[J]. Phytotaxa， 131， 29-34.
WANG WT， PAN KY， LI ZY， 1990. Gesneriaceae [M]// WANG WT. Flora Reipublicae Popularis Sinicae. Beijing： Science Press， 69： 190-203.
WANG WT， PAN KY， LI ZY，et al.， 1998. Gesneriaceae [M]//WU ZY， RAVEN PH. Flora of China. Beijing： Science Press; St. Louis： Missouri Bot Garden， 18： 268-272.