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目的探讨白藜芦醇(resveratrol)对血清饥饿诱导的人胃癌细胞SGC-7901上皮间质转化的影响及机制。方法体外培养胃癌细胞SGC-7901分为对照组(普通培养液,不加白藜芦醇)、白藜芦醇+正常组(普通培养液,加白藜芦醇)、饥饿组(无血清培养液,不加白藜芦醇)、白藜芦醇+饥饿组(无血清培养液,加白藜芦醇),采用MTT法检测各组胃癌细胞凋亡的半抑制浓度,采用细胞划痕试验检测各组胃癌细胞迁移距离,采用实时荧光定量PCR法检测各组胃癌细胞上皮间质转化相关因子Snail、E-cadherin及vimentin mRNA表达情况,采用Western blot法检测各组胃癌细胞Snail、Ecadherin及vimentin蛋白表达情况。结果对照组、饥饿组胃癌细胞凋亡的半抑制浓度分别为(43.90±2.29)、(40.57±1.83)μmol/L;饥饿组胃癌细胞迁移距离[(25.27±0.93)mm]明显大于对照组[(16.20±0.89)mm]、白藜芦醇+正常组[(11.77±0.40)mm]和白藜芦醇+饥饿组[(15.77±0.63)mm](P均<0.05),对照组大于白藜芦醇+正常组(P<0.05);饥饿组细胞Snail mRNA和蛋白(3.07±0.19、1.74±0.04)、vimentin mRNA和蛋白(3.89±0.24、5.06±0.65)表达水平明显高于对照组(Snail mRNA和蛋白分别为1、1,vimentin mRNA和蛋白分别为1、1)、白藜芦醇+正常组(Snail mRNA和蛋白分别为0.65±0.05、0.88±0.03,vimentin mRNA和蛋白分别为0.52±0.02、2.48±0.12)和白藜芦醇+饥饿组(Snail mRNA和蛋白分别为0.62±0.03、0.88±0.02,vimentin mRNA和蛋白分别为0.68±0.05、1.75±0.03)(P均<0.05),E-cadherin mRNA和蛋白表达水平(0.20±0.01、0.24±0.01)明显低于对照组(1、1)、白藜芦醇+正常组(1.69±0.09、1.72±0.07)和白藜芦醇+饥饿组(1.98±0.07、1.43±0.01)(P均<0.05);白藜芦醇+饥饿组细胞Snail、E-cadherin、vimentin mRNA和蛋白水平与对照组、白藜芦醇+正常组比较差异均无统计学意义(P>0.05)。结论白藜芦醇可抑制血清饥饿诱导的人胃癌细胞SGC-7901上皮间质转化,从而抑制胃癌的侵袭、转移。
Objective To investigate the effect of resveratrol on serum starvation induced SGC-7901 epithelial-mesenchymal transition and its mechanism. Methods SGC-7901 gastric cancer cells were divided into control group (normal medium without resveratrol), resveratrol + normal group (normal medium plus resveratrol), starvation group (serum-free medium Liquid, without resveratrol), resveratrol + starvation group (serum-free medium, plus resveratrol), MTT assay was used to detect the semi-inhibitory concentration of gastric cancer cells in each group, using cell scratch test The migration distance of gastric cancer cells in each group was detected. The expressions of Snail, E-cadherin and vimentin in gastric cancer cells were detected by real-time fluorescence quantitative PCR. The expressions of Snail, Ecadherin and vimentin Protein expression. Results The half inhibitory concentrations of gastric cancer cells in control group and starvation group were (43.90 ± 2.29) and (40.57 ± 1.83) μmol / L, respectively. The migration distance of gastric cancer cells in starvation group [(25.27 ± 0.93) mm] (16.20 ± 0.89) mm], resveratrol + normal group (11.77 ± 0.40) mm and resveratrol + starvation group (15.77 ± 0.63) mm] (all P <0.05) The expression of Snail mRNA and protein (3.07 ± 0.19,1.74 ± 0.04), vimentin mRNA and protein (3.89 ± 0.24,5.06 ± 0.65) in starvation group were significantly higher than those in control group (P <0.05) Snail mRNA and protein were 1, 1, vimentin mRNA and protein were 1, 1), resveratrol + normal group (Snail mRNA and protein were 0.65 ± 0.05,0.88 ± 0.03, vimentin mRNA and protein were 0.52 ± 0.02, 2.48 ± 0.12) and resveratrol + starvation group (0.62 ± 0.03,0.88 ± 0.02 for Snail mRNA and protein, respectively), vimentin mRNA and protein were 0.68 ± 0.05 and 1.75 ± 0.03 respectively (all P <0.05) , The expression of E-cadherin mRNA and protein (0.20 ± 0.01,0.24 ± 0.01) was significantly lower than that of the control group (1,1), resveratrol + normal group (1.69 ± 0.09,1.72 ± 0.07) and resveratrol + Starvation group (1.98 ± 0.07,1.43 ± 0.01) (P <0.05). There was no significant difference in the mRNA and protein levels of Snail, E-cadherin and vimentin between resveratrol + starvation group and control group and resveratrol + normal group Significance (P> 0.05). Conclusion Resveratrol can inhibit the serum starvation-induced SGC-7901 epithelial-mesenchymal transition and inhibit the invasion and metastasis of gastric cancer.