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目的探讨临床肝病病人中庚型肝炎病毒（ＧＢＶ－Ｃ／ＨＧＶ）感染情况及临床特点。方法应用庚型肝炎病毒基因组５’ＵＴＲ两对寡核苷酸作为引物，建立逆转录套式聚合酶链反应，检测１６９例不同肝病患者血清标本中ＧＢＶ－Ｃ／ＨＧＶＲＮＡ，并对其中１例ＰＣＲ扩增产物进行克隆及测序。结果１６９例各型肝病病人ＧＢＶ－Ｃ／ＨＧＶＲＮＡ总的检出率为９５％（１６／１６９）。在２９例有手术输血史患者中，３１０％（９／２９）ＧＢＶ－Ｃ／ＨＧＶＲＮＡ呈阳性，明显高于无手术输血史组（５％，Ｐ＜００１）。序列分析显示１株庚肝病毒５’ＵＴＲ部分基因片段与已知庚肝病毒株核苷酸同源性在８９１４％～９８９１％之间。结论ＧＢＶ－Ｃ／ＨＧＶ感染普遍存在于临床肝病患者中，病人感染ＧＢＶ－Ｃ／ＨＧＶ的临床表现未发现有特殊性，ＧＢＶ－Ｃ／ＨＧＶ可能不是非甲～戊型肝炎的主要致病因子。
Objective To investigate the prevalence and clinical features of hepatitis G virus (GV-C / HGV) infection in patients with clinical liver disease. METHODS: Two pairs of oligonucleotides of 5’UTR of Hepatitis G virus were used as primers to establish reverse transcription-polymerase chain reaction (PCR-RT-PCR) and to detect GBV-C / HGVRNA in serum of 169 patients with different liver diseases. One case of PCR Amplification products were cloned and sequenced. Results The total detection rate of GBV-C / HGVRNA in 169 cases of various liver diseases was 95% (16/169). Among 29 patients with transfusion history, 310% (9/29) of GBV-C / HGVRNA were positive, which was significantly higher than that of non-operation transfusion history group (5%, P <001). Sequence analysis showed that the homology between the 5’UTR gene fragment of one strain of Hepatitis G virus and that of the known Ggt strain was between 89.14% and 98.91%. Conclusions GBV-C / HGV infection is common in patients with clinical liver disease. The clinical manifestations of patients infected with GBV-C / HGV were not found to be specific. GBV-C / HGV may not be the major causative agent of non-hepatitis E-hepatitis E virus.