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应用细胞培养和间接免疫荧光法测定脐带血、成人外周血、成人骨髓的Ｔ淋巴细胞集落形成情况及培养前后淋巴细胞亚群的变化。结果表明：脐带血的ＣＦＵ－ＴＬ显著低于成人外周血（Ｐ＜０．０１），略低于成人骨髓（Ｐ＜０．０５）；培养前脐带血的ＣＯ＋３、ＣＯ＋４细胞含量均显著低于成人外周血（Ｐ＜０．０１），与成人骨髓相近似。脐带血的ＣＤ＋８细胞含量与成人外周血和成人骨髓相似（Ｐ＜０．０５）；培养后的ＣＦＵ－ＴＬ中ＣＤ＋３细胞含量脐带血与成人外周血、成人骨髓几乎相同（Ｐ＞０．０５），但其中ＣＤ＋４细胞含量脐带血明显低于成人外周血及成人骨髓（Ｐ＜０．０１），而脐带血的ＣＤ＋８则明显高于成人外周血（Ｐ＜０．０１）和成人骨髓（Ｐ＜０．０５），致使培养后ＣＦＵ－ＴＬ中脐血ＣＤ＋４／ＣＤ＋８显著低于成人外周血和成人骨髓。另培养前的脐带血，成人骨髓中ＣＤ＋４、ＣＤ＋８细胞阳性百分率之和超过ＣＤ＋３百分率，出现ＣＤ＋４、ＣＤ＋８双标记细胞
The formation of T lymphocytes in cord blood, adult peripheral blood and adult bone marrow and the changes of lymphocyte subsets before and after culture were measured by cell culture and indirect immunofluorescence. The results showed that the CFU-TL of umbilical cord blood was significantly lower than that of adult peripheral blood (P <0.01) and slightly lower than that of adult bone marrow (P <0.05). The contents of CO + 3 and CO + 4 in umbilical cord blood were significantly lower Adult peripheral blood (P <0.01), similar to adult bone marrow. The levels of CD + 8 cells in cord blood were similar to those in adult peripheral blood and adults (P <0.05). The levels of CD + 3 cells in CFU-TL after culture were almost the same as those in adult peripheral blood and adults (P> 0.05) , But the CD + 4 cell content of cord blood was significantly lower than that of adult peripheral blood and adult bone marrow (P <0.01), while the cord blood CD + 8 was significantly higher than adult peripheral blood (P <0.01) and adult bone marrow (P < 0.05), resulting in significantly lower CD + 4 / CD + 8 levels in cord blood of CFU-TL than adult peripheral blood and adult bone marrow. Another pre-culture of cord blood, adult bone marrow CD + 4, CD + 8 cells and the percentage of positive than CD + 3 percentage, there CD + 4, CD + 8 double labeled cells