采用含0.5%羧甲基纤维素钠硅胶G薄层色谱分离和CS -930荧光扫描法测定扶正丹中补骨脂素的含量,不受处方中其他成分的干扰 ,方法灵敏,特异性强,操作简便,结果可靠,可作为质量控制的定量标准。该法线性回归方程为Y=42616.86X +11301.91,r=0.996;加标回收率为101.6%;同一样品点样6次测得的RSD为2.25%。 The content of psoralen in Fuzhengdan was determined by thin-layer chromatography with 0.5% sodium carboxymethylcellulose silica gel and CS-930 fluorescence scanning method without interference from other components in the prescription. The method was sensitive and specific, Easy to operate, reliable results, can be used as a quantitative standard of quality control. The normalized regression equation was Y = 42616.86X + 11301.91, r = 0.996; the recovery rate was 101.6%. The RSD measured 6 times with the same sample was 2.25%.