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目的:探讨HBV-DNA与乙肝两对半检测结果的相关性。方法:选取300例疑似乙肝患者为观察对象,采用荧光定量PCR(FQ-PCR)检测患者血清中的HBV-DNA,两对半指标的检测采用ELISA方法,观察分析结果的相关性,总结经验。结果:检测结果显示,大三阳组(HBsAg(+)HBeAg(+)HBcAb(+))患者血清HBV-DNA检出率为98.3%,平均拷贝数为1.83E+06/ml;小三阳组(HBsAg(+)HBeAb(+)HBcAb(+))患者血清HBV-DNA检出率为64.7%,平均拷贝数为1.81E+03/ml;HBsAg(+)HBcAb(+)组血清HBV-DNA检出率为63.5%,平均拷贝数7.95E+03ml。结论:HBV-DNA水平与HBV M的表现模式存在关联,HBsAg(+)HBeAg(+)HBcAb(+)的标本HBV-DNA值较HBsAg(+)HBeAb(+)HBcAb(+)与HBsAg(+)HBcAb(+)的标本更高,说明HBeAg会影响到HBV-DNA的水平。FQ-PCR对于准确定量和检测HBV感染复制情况的检测都有很高的应用价值,对于乙型肝炎的诊断和治疗都具有推广价值。
Objective: To investigate the relationship between HBV-DNA and hepatitis B two-and-a-half test. Methods: Three hundred patients with suspected hepatitis B were selected as the observation subjects. HBV-DNA in sera of patients was detected by fluorescence quantitative PCR (FQ-PCR). ELISA was used to detect the two pairs of semi-indexes. The correlation between the results and the experience was summarized. Results: The detection results showed that the detection rate of HBV-DNA in the patients with HBsAg (+) HBeAg (+) HBcAb (+) was 98.3% and the average copy number was 1.83E + 06 / ml. (+) HBcAb (+)), the positive rate of HBV-DNA in serum of HBsAg (+) HBcAb (+) group was 64.7% and the average copy number was 1.81E + The detection rate was 63.5% with an average copy number of 7.95E + 03ml. Conclusion: The HBV-DNA level is correlated with the expression pattern of HBV M, and the HBV-DNA value of HBsAg (+) HBeAg (+) HBcAb (+) is higher than that of HBsAg (+) HBeAb (+) HBcAb ) Higher HBcAb (+) specimens indicate that HBeAg affects HBV-DNA levels. FQ-PCR for the accurate quantitative detection of HBV replication and detection of detection have a high value, for the diagnosis and treatment of hepatitis B has a promotional value.