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目的 探讨肝癌细胞分化的特异指标。方法 用公认的细胞分化诱导剂维甲酸 (RA)、丁酸钠 (SB)和肝细胞毒剂四氯化碳处理 BEL- 740 2人肝癌细胞 ,观察细胞增殖、软琼脂集落形成率、核质比例和各项生化指标的变化。结果 1 0 μmol/L RA、2 .5mmol/L SB和 1 mmol/L CCl4 均显著抑制肝癌细胞增殖 ,并使其软琼脂集落形成率明显减少 (P<0 .0 5) ,但 RA和 SB处理后 ,细胞核质比例明显减小 (P<0 .0 1或 P<0 .0 5) ,鸟氨酸氨基甲酰转移酶 (OCT)、酪氨酸 -α-酮戊二酸转氨酶 (TAT)和碱性磷酸酶 (ALP)比活力明显升高 ,AFP分泌量和 -谷氨酰转肽酶 ( - GT)比活力明显下降 (P<0 .0 1或 P<0 .0 5) ,而 CCl4 对核质比例和上述生化指标的影响则与 RA和 SB相反。结论 细胞核质比例、AFP分泌量、TAT、OCT和 ALP比活力可作为判断肝癌细胞分化的有用指标 ,其变化与毒性反应有明显的区别。
Objective To investigate the specific markers of hepatoma cell differentiation. METHODS: BEL-740 2 human hepatocellular carcinoma cells were treated with retinoic acid (RA), sodium butyrate (SB) and hepatocyte cytotoxic agent, and cell proliferation, soft agar colony formation rate, and ratio of nuclear to cytoplasm were observed. And changes in various biochemical indicators. Results The proliferation of HCC cells was significantly inhibited by 10 μmol/L RA, 2.5 mmol/L SB and 1 mmol/L CCl4, and the soft agar colony formation rate was significantly reduced (P<0.05), but RA and SB After treatment, the ratio of nucleus to cytoplasm was significantly reduced (P<0.01 or P<0.05), ornithine carbamoyl transferase (OCT), tyrosine-alpha-ketoglutarate transaminase (TAT) The specific activity of alkaline phosphatase (ALP) was significantly increased, and the AFP secretion and the specific activity of -glutamyl transpeptidase (-GT) decreased significantly (P<0.01 or P<0.05). The effect of CCl4 on the ratio of nucleoplasm and the above biochemical indicators is opposite to that of RA and SB. Conclusion The ratio of nucleus to cytoplasm, secretion of AFP, specific activity of TAT, OCT and ALP can be used as a useful index for judging the differentiation of hepatoma cells. The changes are significantly different from those of toxicity.