论文部分内容阅读
目的:观察醒脑液早期干预对阿尔茨海默病模型APPswe/PS△E9双转基因小鼠行为学及脑中胆碱能系统相关酶的影响。方法:将75只PAP双转基因小鼠随机随机分为空白对照组(模型组)、阳性对照组、用药高剂量组、用药中剂量组和用药低剂量组,每组各15只。15只遗传背景相同的转基因阴性的小鼠作为正常动物组。所试药物稀释至相同体积灌胃给药,正常动物组及空白对照组给以等体积蒸馏水灌胃,连续灌胃4个月。采用Morris水迷宫测定小鼠空间学习记忆能力,避暗箱测定其条件学习记忆能力。使用比色法检测小鼠脑中乙酰胆碱(Ach)、乙酰胆碱转移酶(ChAT)及乙酰胆碱酯酶(AchE)的活性。结果:与正常组比较,模型组小鼠的学习记忆能力均有所下降,脑组织中Ach、ChAT、AchE的含量均下降。与模型组比较,阳性对照组、用药高剂量组及用药中剂量组小鼠血浆中Ach含量均有所提高,而AchE含量则出现下降。结论:醒脑液能改善阿尔茨海默病模型小鼠的学习记忆能力,其作用机制可能与调控胆碱能系统有关。
OBJECTIVE: To observe the effect of Xingnong solution on behavior and cholinergic system-related enzymes in APPswe / PSΔE9 double transgenic mice with Alzheimer’s disease. Methods: 75 PAP double transgenic mice were randomly divided into blank control group (model group), positive control group, high dose group, middle dose group and low dose group, 15 in each group. Fifteen transgenic mice with the same genetic background were used as the normal animal group. The drugs tested were diluted to the same volume intragastric administration, normal animal group and blank control group were given equal volume of distilled water, continuous gavage for 4 months. The spatial learning and memory ability of mice was measured by Morris water maze, and the conditions of learning and memory were tested by dark box. The activity of acetylcholine (Ach), acetylcholinesterase (ChAT) and acetylcholinesterase (AchE) in mouse brain were detected by colorimetric method. Results: Compared with the normal group, the learning and memory abilities of the model group decreased and the content of Ach, ChAT and AchE in the brain tissue decreased. Compared with the model group, the Ach content in the plasma of the positive control group, the high dose group and the medium dose group increased, while the AchE content decreased. Conclusion: Xingnao liquid can improve the learning and memory ability of Alzheimer’s disease model mice, which may be related to the regulation of cholinergic system.