目的:建立同时测定白花蛇舌草中去乙酰车草酸酐、车叶草苷、秦皮乙素、4-香豆酸、阿魏酸、槲皮素和山奈酚7种有效成分含量的高效液相色谱方法。方法:采用Thermo ODS-2 HYPERSIL C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为0.05%醋酸水溶液(A)-0.05%醋酸乙腈(B),线性梯度洗脱[0~50 min,5%B#80%B],流速为1.0 ml·min~(-1);柱温为35℃;检测波长分别为238 nm(乙酰车草酸酐和车叶草苷)、347 nm(秦皮乙素)、306 nm(4-香豆酸)、322 nm(阿魏酸)、369 nm(槲皮素和山奈酚)。结果:去乙酰车草酸酐、车叶草苷、秦皮乙素、4-香豆酸、阿魏酸、槲皮素和山奈酚的浓度分别在2.940~58.700μg·ml~(-1),2.650~52.900μg·ml~(-1),0.762~15.200μg·ml~(-1),0.448~8.960μg·ml~(-1),0.794~15.900μg·ml~(-1),0.897~17.900μg·ml~(-1),0.489~9.780μg·ml~(-1)范围内均呈良好线性关系(r≥0.999 7);日内、日间精密度良好,RSD均小于1.95%(n=6);各组分低、中、高浓度的平均加样回收率均在98.16%~101.88%之间,RSD均小于1.88%(n=6);白花蛇舌草中去乙酰车草酸酐、车叶草苷、秦皮乙素、4-香豆酸、阿魏酸、槲皮素和山奈酚含量分别为(42.48±1.43)μg·g~(-1)、(63.76±1.01)μg·g~(-1)、(1 765±0.69)μg·g~(-1)、(881.9±0.74)μg·g~(-1)、(86.99±1.65)μg·g~(-1)、(1 395±0.731)μg·g~(-1)和(902.2±0.82)μg·g~(-1)。结论:本文方法简便可靠,重复性好,可全面反映白花蛇舌草中各种极性较高的成分的含量,适用于白花蛇舌草质量控制。 OBJECTIVE: To establish an HPLC method for the simultaneous determination of seven effective components of deacetyl-oxalic anhydride, carotenoid, aesculetin, 4-coumarate, ferulic acid, quercetin and kaempferol in Hedyotis diffusa Chromatographic methods. METHODS: The chromatographic column (250 mm × 4.6 mm, 5 μm) was used on a Thermo ODS-2 HYPERSIL C 18 column with a mobile phase of 0.05% acetic acid in water (A) 50 min, 5% B # 80% B] at a flow rate of 1.0 ml · min -1 .The column temperature was 35 ℃ .The detection wavelength was 238 nm (acetyl car oxalic anhydride and carotinoside), 347 nm (Aesculetin), 306 nm (4-coumaric acid), 322 nm (ferulic acid), 369 nm (quercetin and kaempferol). Results: The concentrations of deacetyl-oxalic anhydride, carlover glycoside, aesculetin, 4-coumaric acid, ferulic acid, quercetin and kaempferol were 2.940-58.700μg · ml -1, 2.650 ~ 52.900μg · ml -1, 0.762 ~ 15.200μg · ml -1, 0.448 ~ 8.960μg · ml -1, 0.794 ~ 15.900μg · ml -1, 0.897 ~ 17.900 (r≥0.9997) in the range of 0.489 ~ 9.780μg · ml ~ (-1). The intra-day and inter-day precision was good with RSDs less than 1.95% (n = 6). The average recoveries of low, medium and high concentration of each component were between 98.16% and 101.88%, RSD was less than 1.88% (n = 6) The content of carrageenin, aesculetin, 4-coumaric acid, ferulic acid, quercetin and kaempferol were (42.48 ± 1.43) μg · g -1, (63.76 ± 1.01) μg · g -1 ~ (-1), (1765 ± 0.69) μg · g -1, (881.9 ± 0.74) μg · g -1, (86.99 ± 1.65) μg · g -1, ( 1 395 ± 0.731) μg · g -1 and (902.2 ± 0.82) μg · g -1, respectively. Conclusion: This method is simple and reliable, good repeatability, can fully reflect the content of various components with high polarity in Hedyotis diffusa, suitable for the quality control of Hedyotis diffusa.