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采用田间小区试验研究“推荐配比”(N:P2O5:K2O比率为190:90:100 kg hm-2)、“高氮量配比”(N:P2O5:K2O比率为210:90:100 kg hm-2)、“低氮量配比”(N:P2O5:K2O比率为170:90:100 kg hm-2)以及“常规配比”(N:P2O5:K2O比率为216:112.5:202.5 kg hm-2)4种氮、磷、钾配比施肥对饲用稻威优198蔗糖合成酶(SUS)、腺苷二磷酸焦磷酸化酶(AGPase)、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)以及产量和糙米蛋白质的影响。结果表明:“推荐配比”能提高不同生育时期水稻功能叶(旗叶)和粒籽中碳、氮代谢关键酶的活性,这些关键酶活性的变化显著影响水稻产量和糙米全氮以及蛋白氮的含量。统计(P0.05)结果证实“推荐配比”能提高水稻产量达到8200 kg hm-2,与“常规配比”相比产量提高了24.81%;“推荐配比”糙米全氮和蛋白氮含量分别达到22.70 g kg-1和21.98 g kg-1,与“常规配比”相比差异显著,并且其全氮和蛋白氮含量分别提高17.01%,18.38%。
The ratio of N: P2O5: K2O (210: 90: 100 kg) was determined by the field trial of “recommended ratio” (N: P2O5: K2O ratio of 190: 90: 100 kg hm-2) (N: P2O5: K2O ratio of 216: 112.5: 202.5 kg / hm-2) and low nitrogen ratio (N: P2O5: K2O ratio of 170: 90: 100 kg hm-2) hm-2). The effects of four nitrogen, phosphorus and potassium fertilizers treatments on the effects of different fertilizers on the activities of sucrose synthase (SUS), adenosine diphosphate pyrophosphorylase (AGPase), nitrate reductase (NR), glutamine Synthetase (GS) and yield and brown rice protein. The results showed that the “recommended proportioning” could increase the activities of key enzymes of carbon and nitrogen metabolism in functional leaves (flag leaf) and grain seed of different growth stages. The changes of these key enzyme activities significantly affected the yield of rice and the content of total nitrogen and protein nitrogen in brown rice Content. Statistics (P <0.05) confirmed that the “recommended ratio” could increase the yield of rice to 8200 kg hm-2 and increase the yield by 24.81% compared with the “conventional proportion”. The “recommended ratio” of total nitrogen and protein nitrogen in brown rice Reaching 22.70 g kg-1 and 21.98 g kg-1, respectively. Compared with the “conventional compounding”, the difference was significant, and the content of total nitrogen and protein nitrogen increased by 17.01% and 18.38% respectively.