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目的 观察Vβ13 mRNA和Treg细胞在大鼠细小病毒(KRV)联合巨细胞病毒(CMV)感染大鼠的脾淋巴细胞中的表达以及海藻提取物对1型糖尿病的治疗效果.方法 LEW.1WR1大鼠40只,随机分成2组,应用CMV联合KRV建立大鼠1型糖尿病模型.治疗组大鼠每日口服海藻提取物共40 d;成模组用等量无菌生理盐水替代同步进行.RT-PCR检测脾脏Vβ13 mRNA的表达,流式细胞术检测脾细胞Vβ13+ CD4+CD25+ Treg细胞亚群比例;HE染色观察糖尿病胰岛的病变情况.结果 实验组成模率为90％ (18/20),成模血糖平均水平在3.15 g/L;实验组Vβ13的表达高于治疗组.实验组中CD4+ CD25+ Treg细胞的比例为6.2％,治疗组为13.5％ (P＜0.05).结论 感染巨细胞病毒的大鼠Vβ13 mRNA的表达在糖尿病初期显著增高.Vβ13+ CD4+ CD25+ Treg细胞亚群比例较低.海藻提取物提高了LEW.1WR1大鼠Treg细胞的表达,可用于治疗糖尿病.“,”Objective To measure the expression of Vβ13 mRNA and Treg cells in spleen lymphocytes from rats infected with cytomegalovirus (CMV) as well as Kilham rat virus (KRV) and the therapeutic effects of seaweed extract on type 1 diabetes mellitus.Methods Forty LEW.1WR1 rats were randomly divided into two groups.The rat model of type 1 diabetes mellitus was established using rat cytomegalovirus combined with KRV.The rats in the treatment group were treated with seaweed extract daily for 40 days,while the experimental group was given the same amount of sterile saline.The expression of Vβ13 mRNA in the spleen was assessed by RT-PCR and CD4+ CD25+ Treg cells were detected by flow cytometry (FCM).The sections in splenic cells were detected by HE staining.Results In the experimental group,90％ of the rats were diabetic (18/20),and the mean blood glucose level was 3.15 g · L1.The expression of Vβ13 in the experimental group was higher than that in the treatment group.The ratio of CD25+ Treg cells in the experimental group was 6.2％ and in the treatment group 13.5％ (P＜0.05).Conclusion The expression of Vβ13 mRNA was significantly higher in the early stage of virus-induced diabetes,and the proportion of CD4+ CD25+ Treg cells was lower in the experimental group.Seaweed extract increased the Treg cell expression for the treatment of diabetes.