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目的:建立康乐洗液中蛇床子素的含量测定方法。方法:高效液相色谱法。采用Symmetry C18柱(3.9 mm×150mm,5μm),流动相为乙腈-水(65∶35),检测波长为322 nm。结果:此方法线性关系良好,平均加样回收率为99.88%。结论:此方法简便、准确,可用于测定康乐洗液中蛇床子素的含量。
Objective: To establish a method for determination of osthole content in lotion. Methods: High performance liquid chromatography. A Symmetry C18 column (3.9 mm × 150 mm, 5 μm) was used. The mobile phase was acetonitrile-water (65:35) and the detection wavelength was 322 nm. Results: The linearity of this method was good with the average recovery of 99.88%. Conclusion: This method is simple and accurate and can be used to determine the content of Osthole in Lotion lotion.