Structural biology aims to provide structural insights into our understandings of biological processes at the atomic level.Atomic structures of biological macromolecules are often determined by one of the three major methods:X-ray crystallography,NMPR spectroscopy and cryo-electron microscopy (cryoEM) or,more specifically,single-particle cryo-EM.While X-ray crystallography has contributed most of the atomic structures in the protein databank,recent technological breakthroughs in singleparticle cryo-EM have changed landscape of structural biology in a dramatic and revolutionary manner [1].Many challenging biological questions that were considered too difficult to be studied structurally even a few years ago have now been,or are being,studied by single-partide cryo-EM,providing novel insights into our understandings of many important biological processes at the molecular and atomic levels [2].