我院1994年6月～1997年5月对142例肾移植患者术后痰液培养，细菌和真菌检出情况报告如下。材料与方法一、痰液标本采集清晨首先让患者用无菌生理盐水或温开水反复漱口，轻咳弃去第一口痰，再深咳1～2口，痰液直接吐入消毒大口试管内，立即送检。二、细菌和真菌的分离与鉴定1． Our hospital from June 1994 to May 1997 on 142 cases of renal transplant patients sputum culture, the detection of bacteria and fungi are as follows. Materials and methods First, the sputum specimens collected early in the morning the patient with sterile saline or warm water repeatedly gargle, light cough to go to the first phlegm, then deep cough 1 to 2 mouth, sputum directly into the disinfection of large mouth test Tube, immediately seized. Second, the separation and identification of bacteria and fungi 1.