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[Objective] To obtain the optimum medium formula for proliferation of D. candidum protocorm. [Method] The optimum concentration ratio of 6-BA, NAA and KT was screened by orthogonal test, and effect of each factor on the proliferation of D. candidum protocorm was investigated. [Result] The optimum medium formula was: 1/2MS+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L KT. [Conclusion] The study provides basis for the industrialized production of protocorm and breeding high quality D. candidum.
[Objective] To obtain the optimum medium formula for proliferation of D. candidum protocorm. [Method] The optimum concentration ratio of 6-BA, NAA and KT was screened by orthogonal test, and effect of each factor on the proliferation of D. candidum [Result] The study provides basis for the industrialized production of protocorm. [Result] The optimum medium formula was: 1 / 2MS + 2.0 mg / L 6-BA + 0.5 mg / L NAA + 1.0 mg / and breeding high quality D. candidum.