论文部分内容阅读
中美双方为探讨适用于进口粮检疫的小麦矮腥黑穗病菌(Tilletia controversa Kuehn,以下简称TCK)准确简便的鉴定方法,于1989～1992年进行联合试验。本文以美国不同时期和不同地域所收集的小麦矮腥黑穗病菌(TCK)和小麦网腥黑穗病菌(T.caries Tul.,以下简称TCT)共135个菌株为材料,对这两种病菌的自发荧光显微学特性,作了比较研究,建立了数学模型,并在人工污染样品中进行了应用研究,结果表明,自发荧光显微学法和LI-F方程,可以有效地鉴别TCK或TCT菌株,但如应用于鉴别进口粮中混合的或少量的TCK、TCT冬孢子,则其准确率随孢子含量的下降而降低。
Both China and the United States conducted a joint test from 1989 to 1992 to explore the accurate and convenient identification method for Tilletia controversa Kuehn (hereinafter referred to as TCK) for imported food quarantine. In this paper, a total of 135 strains of Triticum aestivum (TCK) and T.caries Tul. (TCT) collected in different periods and regions in the United States were used as materials. The results of the autofluorescence microscopy and the LI-F equation can be used to identify the TCK or TCT strains, but if used to identify mixed or small amounts of TCK and TCT teliospores mixed with imported grain, their accuracy decreases with decreasing spore content.