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采用直接法和间接法进行了９９ｍＴｃ标记重组水蛭素的研究，结果表明，间接法制备的９９ｍＴｃ－重组水蛙素具有更高的放化纯和稳定性。更适合于血栓模型的显像研究。９９ｍＴｃ－重组水蛙素在小鼠体内的分布表明，心、肝、脾、肺和脑的放射性分布均较低；放射性主要集中在肾脏，１５ｍｉｎ时肾脏放射性可达总注入剂量的６５％；且血液中放射性清除较快。兔血栓模型显像结果表明，９９ｍＴｃ－重组水蛙素可在血栓部位浓聚；注入后１ｈ即可使血栓清晰显影。因此，９９ｍＴｃ－重组水蛙素有望成为一种新型的血栓显像剂。
The direct method and indirect method were used to study the 99mTc-labeled recombinant hirudin. The results showed that the 99mTc-recombinant water-frog has higher radiochemical purity and stability. More suitable for thrombus model imaging studies. The distribution of 99mTc-recombinant water frog in mice showed that the distribution of radioactivity in heart, liver, spleen, lung and brain was low. The radioactivity was mainly localized in the kidneys. At 15 minutes, the renal radioactivity reached 65% of the total injected dose. Radioactive clearance in the blood is faster. The results of rabbit thrombus model showed that 99mTc-recombinant water Fogan could accumulate in the thrombus site; thrombus could be clearly developed at 1h after injection. Therefore, 99mTc-recombinant water frog is expected to become a new thrombotic imaging agent.