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利用GeneFishing技术获得耐低温花生品种花育44号种子在常温与低温诱导下的差异表达基因(differentially expressed genes,DEGs)ACP20和ACP2。BLAST比对后发现ACP20基因与花生油质蛋白基因同源性达99%,ACP2基因与花生nifU-like(铁硫簇蛋白)蛋白mRNA同源性达99%,说明油质蛋白和nifU-like蛋白可能与花生对低温逆境胁迫的抗性有关。通过RACE技术从该品系获得相关基因的完整编码区,对其进行生物学功能预测,为探讨花生耐低温分子机制乃至培育耐低温花生新品种奠定了基础。
GeneFishing was used to obtain differentially expressed genes (DEGs) ACP20 and ACP2 in Hua Yu 44, a peanut variety with low temperature tolerance. BLAST showed that the homology between ACP20 gene and peanut oleosin gene was 99%, and the homology between ACP2 gene and peanut nifU-like mRNA was 99%, which indicated that oleosin and nifU-like protein It may be related to the resistance of peanut to low temperature stress. The complete coding region of the related gene was obtained from the line by RACE technology and its biological function was predicted. This laid the foundation for exploring the molecular mechanism of low temperature resistance of peanut and even cultivating the new low temperature resistant peanut.