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应用原位末端标记（ＩＳＥＬ）ＤＮＡ 片断技术和增殖细胞核抗原（ＰＣＮＡ） 免疫组化染色方法，观察鼻咽癌（ＮＰＣ） 及其癌旁粘膜上皮的细胞凋亡和细胞增殖。结果显示：①ＮＰＣ 中可见片状分布的ＩＳＥＬ 阳性细胞，其形态不表现凋亡特征，以泡状核细胞癌更常见。②ＮＰＣ 的ＩＳＥＬ 阳性率及ＩＳＥＬ 染色强度指数（ＳＩＩ） 高于癌旁粘膜（ Ｐ ＜ ０ ．０５） ；ＮＰＣ 的ＩＳＥＬ ＳＩＩ 与ＰＣＮＡ ＳＩＩ 呈正相关（ ｒ ＝ ０ ．３４１ ， Ｐ ＜ ０ ．０５） ，但其ＩＳＥＬ 阳性率及ＳＩＩ均低于ＰＣＮＡ（ Ｐ ＜ ０ ．０１） 。③泡状核细胞癌ＩＳＥＬＳＩＩ和ＰＣＮＡＳＩＩ均高于低分化鳞癌（ 分别为Ｐ ＜ ０ ．０１ ， Ｐ ＜ ０ ．０５） ，前者５ 年生存率相对较高。提示ＮＰＣ 细胞增殖与细胞凋亡有关。
The apoptosis and cell proliferation of nasopharyngeal carcinoma (NPC) and its para-cancerous mucosa epithelium were observed by ISEL DNA fragmentation and PCNA immunohistochemical staining. The results showed that: (1) ISEL-positive cells with platelet distribution were visible in the NPC. The morphological features of the ISEL-positive cells were not characterized by apoptosis, but were more common in the so-called goblet cells. (2) ISEL positive rate and ISEL staining index (SII) in NPC were significantly higher than those in para-cancerous mucosa (P <0.05); ISEL SII in NPC was positively correlated with PCNA SII (r = 0.341, P <0.05) The ISEL positive rate and SII were lower than PCNA (P <0 .01). ③ The positive rate of ISELSII and PCNASII were higher in poorly differentiated squamous cell carcinoma (P <0.01, P <0.05, respectively). The 5-year survival rate of the former was relatively high. Prompted NPC cell proliferation and apoptosis related.