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目的探讨5-羟色胺2A(5-HT2A)受体对新生大鼠延髓脑片吸气神经元放电的调制作用。方法制作新生大鼠离体延髓脑片标本,给予灌流恒温改良Kreb′s液(MKS),使用内含银-氯化银电极的吸附电极吸附舌下神经根,稳定记录舌下神经根呼吸相关节律性放电活动(RRDA)后,使用微电极以细胞外记录方式在面神经后核内侧区同步记录吸气神经元放电。同步记录舌下神经根RRDA和吸气神经元放电后,用含40μmol/L5-HT2A受体特异激动剂2,5-二甲氧基-4-碘苯基丙烷-2-胺盐酸盐(DOI)的MKS充分灌流延髓脑片,记录吸气神经元放电活动变化,冲洗至RRDA和吸气神经元放电基本恢复,用含5-HT2A受体特异拮抗剂酮舍林的MKS灌流脑片,记录吸气神经元放电活动变化。结果使用DOI激活5-HT2A受体后,吸气神经元放电时程(TI)为(0.86±0.07)s,呼气时程(TE)为(10.78±1.06)s,呼吸周期(RC)为(11.79±1.64)s,吸气神经元放电积分幅度(IA)为(357.98±37.21)(μV.s),放电峰频率(PF)为(37.83±3.66)Hz,对照组分别为(0.68±0.06)s、(13.89±2.14)s、(14.77±1.92)s、(273.57±24.39)(μV.s)和(29.92±4.50)Hz,DOI组与对照组比较均有显著差异(Pa<0.01)。对吸气神经元放电有兴奋作用。应用酮舍林后,缩短TI为(0.55±0.07)s,延长TE为(18.43±3.28)s,RC为(20.17±2.91)s,IA减为(214.37±33.52)(μV.s),PF为(22.17±3.92)Hz,与对照组和DOI组比较均有显著差异(Pa<0.01)。对吸气神经元放电有抑制作用。结论5-HT2A受体参与调节新生大鼠延髓脑片吸气神经元放电活动。
Objective To investigate the modulation of 5-hydroxytryptamine 2A (5-HT2A) receptor on inspiratory neurons in medulla oblongata of neonatal rats. Methods The isolated medulla oblongata slice of neonatal rats was made. The Krebs liquid (MKS) modified by perfusion was given to the neonatal rats. The sublingual nerve roots were adsorbed by the adsorption electrodes containing silver-silver chloride electrode. The sublingual nerve roots were stably recorded After rhythmic discharge activity (RRDA), inhalation neurons were synchronously recorded in the medial area of the posterior nucleus of the facial nerve by extracellular recording using a microelectrode. Simultaneous recording of the hypoglossal nerve root RRDA and the inspiratory neuron discharge, the cells were treated with 40 μmol / L 5-HT2A receptor specific agonist 2,5-dimethoxy-4-iodophenylpropan-2-amine hydrochloride DOI) was used to perfuse medulla oblongata slices. The discharge activity of inspiratory neurons was recorded. After rinsing to RRDA and discharge of inspiratory neurons, recovery was basically achieved. MKS perfusion slices containing ketamine, a specific antagonist of 5-HT2A, Record inhalation neuron discharge activity changes. RESULTS: After inhalation of 5-HT2A receptor using DOI, the inhalation neuron discharge time (TI) was (0.86 ± 0.07) s, the expiratory time was (10.78 ± 1.06) s and the respiratory cycle (11.79 ± 1.64) s, the discharge integral amplitude (IA) of inspiratory neurons was (357.98 ± 37.21) μV.s and the peak discharge frequency was (37.83 ± 3.66) Hz, while the control group was (0.68 ± 0.06) s, (13.89 ± 2.14) s, (14.77 ± 1.92) s, (273.57 ± 24.39) (μV.s) and (29.92 ± 4.50) Hz, respectively. There was significant difference between DOI group and control group ). Exciting neurons are excited about the discharge. The shortened TI was (0.55 ± 0.07) s, the extended TE was (18.43 ± 3.28) s, the RC was (20.17 ± 2.91) s and the IA was reduced to (214.37 ± 33.52) (22.17 ± 3.92) Hz, there was a significant difference (P <0.01) between the control group and the DOI group. On inhalation neuron discharge inhibition. Conclusions 5-HT2A receptors are involved in the discharge activity of inspiratory neurons in medulla oblongata of neonatal rats.