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目的研究脂联素基因(adiponectin gene,ap M1 gene)第2外显子(Exon2)单核苷酸多态性(single nucleotide polymorphism,SNP)与儿童单纯性肥胖的关系,为内蒙古汉族儿童单纯性肥胖的研究与防治提供参考,为疾病的防治提供新的治疗靶点。方法采用整群抽样方法,于2015年9-10月选取内蒙古包头市汉族100例肥胖儿童和100例正常体重儿童。利用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)方法对ap M1Exon2 SNP+45(T→G)多态位点进行基因测定,计算等位基因频率,并且分析其与肥胖代谢的关联性。同时进行身高、体重、血清空腹血糖(FPG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)的测定。用SPSS 17.0软件进行t检验、χ~2检验。结果肥胖组和正常体重组间FPG、TC、TG、HDL-C和LDL-C水平差异均有统计学意义(P<0.01)。肥胖组和正常体重组ap M1Exon2 SNP+45(T→G)多态位点GG型、TG型、TT型基因型频率分别为14.0%、56.0%、30.0%和4.0%、42.0%、54.0%,基因型频率符合Hardy-Weinberg遗传平衡定律。G、T两组等位基因频率分别为42.0%、58.0%和25.0%、75.0%,两组基因型频率与等位基因频率分布差异有统计学意义(P<0.05)。在肥胖组,SNP+45(T→G)多态位点TG+GG基因型与TT型相比,其FPG、TG、TC、LDL-C水平升高,而HDL-C水平降低,差异均有统计学意义(P<0.05);在正常体重组,TG+GG基因型的FPG、TC较TT型低,差异均有统计学意义(P<0.05)。结论 ap M1Exon2 SNP+45(T→G)单核苷酸多态性与包头市100例汉族儿童单纯性肥胖可能有关。
Objective To investigate the relationship between Exon2 single nucleotide polymorphism (SNP) of adiponectin gene (ap M1 gene) and childhood simple obesity in Han nationality in Inner Mongolia. Provide a reference for the research and prevention of obesity, and provide a new therapeutic target for the prevention and treatment of diseases. Methods By cluster sampling method, 100 obese children and 100 normal weight children from Han nationality in Baotou, Inner Mongolia were selected from September to October in 2015. The polymorphism of ap M1Exon2 SNP + 45 (T → G) was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the frequency of alleles was calculated and compared with that of obesity metabolism The relevance of. At the same time, the body weight, serum fasting blood glucose (FPG), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL- SPSS 17.0 software t test, χ ~ 2 test. Results The levels of FPG, TC, TG, HDL-C and LDL-C in obesity group and normal weight group were significantly different (P <0.01). The frequencies of GG, TG and TT genotypes in ap M1Exon2 SNP + 45 (T → G) polymorphism sites in obesity group and normal body weight group were 14.0%, 56.0%, 30.0% and 4.0%, 42.0% and 54.0% , Genotype frequencies in line with the Hardy-Weinberg law of genetic equilibrium. The allele frequencies in G and T groups were 42.0%, 58.0% and 25.0%, 75.0%, respectively. There was significant difference in genotype frequency and allele frequency between the two groups (P <0.05). In obese group, TG + GG genotype of SNP + 45 (T → G) polymorphism increased in FPG, TG, TC, LDL-C and HDL-C compared with TT (P <0.05). In normal weight group, FPG and TC of TG + GG genotype were lower than TT genotype (P <0.05). Conclusion The SNP + 45 (T → G) polymorphism of ap M1Exon2 may be related to the simple obesity of 100 Han children in Baotou.