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目的检测HBV前S1蛋白(PreS1-Ag)的水平,分析与HBV DNA复制的关系。方法用酶联免疫吸附分析法(ELISA)检测HBV感染者血清中PreS1-Ag和HBV血清标志物(HBsAg、HBsAb、HBeAg、HBeAb、HBcAb),同时用Real Time PCR检测血清中HBV DNA含量。结果 407例HBV感染者HBeAg(+)的大三阳模式135例血清中,HBV-DNA、PreS1-Ag阳性率分别为87.4%和81.5%,两者差异无统计学意义;在117例HBeAg(-)的小三阳模式血清中,HBV-DNA、PreS1-Ag阳性检出率分别为74.4%和69.2%,两者差异无统计学意义。在266例HBV-DNA阳性患者血清中,PreS1-Ag阳性和HBeAg阳性检出率分别为74.4%、60.2%,两者差异具有统计学意义,P<0.01。结论 PreS1-Ag与HBV-DNA检测结果一致性较好,较HBeAg能更灵敏地反映体内HBV的复制状况,可与HBV-DNA互相补充,在临床诊断和治疗HBV感染中有重要意义。
Objective To detect the level of PreS1-Ag and analyze the relationship with HBV DNA replication. Methods Serum PreS1-Ag and HBV serum markers (HBsAg, HBsAb, HBeAg, HBeAb, HBcAb) were detected by enzyme-linked immunosorbent assay (ELISA). HBV DNA levels in serum were detected by Real Time PCR. Results The positive rates of HBV-DNA and PreS1-Ag in 87 cases of HBeAg (+) HBeAg positive serum samples from patients with HBV infection were 87.4% and 81.5%, respectively, with no significant difference between the two groups. Among the 117 HBeAg -) of the small three positive model serum, HBV-DNA, PreS1-Ag positive detection rates were 74.4% and 69.2%, no significant difference between the two groups. The positive rate of PreS1-Ag and HBeAg in serum of 266 HBV-DNA positive patients was 74.4% and 60.2%, respectively, with statistical significance (P <0.01). Conclusions The results of PreS1-Ag and HBV-DNA are in good agreement. Compared with HBeAg, it can reflect the replication status of HBV in vivo more easily and complement with HBV-DNA, which is of great significance in clinical diagnosis and treatment of HBV infection.