为研究外源性Ｒｂ 和Ｐ５３ 基因导入血管平滑肌细胞后对ｃｆｏｓ 和ｃｊｕｎ 基因表达的调控作用， 将Ｒｂ 基因或Ｐ５３ 基因重组腺病毒载体转染人脐动脉血管平滑肌细胞， 应用ＲＴＰＣＲ 和免疫组化法检测ｃｆｏｓ、ｃｊｕｎ 的ｍＲＮＡ及蛋白质表达水平， 以ＤＮＡ 琼脂糖凝胶电泳和β半乳糖苷酶染色分别观察细胞凋亡和细胞衰老． 结果显示， 野生型Ｐ５３ 基因导入可诱导平滑肌细胞凋亡，ｃｆｏｓ 和ｃｊｕｎ ｍＲＮＡ 及蛋白质表达水平显著增高． 外源性Ｒｂ 基因导入能促进细胞衰老， 下调ｃｆｏｓ 基因表达， 但对ｃｊｕｎ 基因表达没有明显影响 In order to study the regulatory effect of exogenous Rb and P53 gene on vascular smooth muscle cells after c  fos and c  jun gene expression, the Rb gene or P53 gene recombinant adenovirus vector transfected human umbilical artery smooth muscle cells, using RT  PCR and immunohistochemistry c  fos, c  jun mRNA and protein expression levels, DNA agarose gel electrophoresis and β  galactosidase staining were observed apoptosis and cell senescence. The results showed that the wild-type P53 gene can induce apoptosis of smooth muscle cells, c  fos and c  jun mRNA and protein expression levels were significantly higher. Exogenous Rb gene can promote cell senescence, down c  fos gene expression, but c  jun gene expression was not significantly affected