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目的：研究５０ Ｈｚ 工频磁场对细胞胞浆蛋白质酪氨酸磷酸化的影响，以初步探讨工频磁场生物效应有关细胞信息转导的机制。方法：对分别经两个不同强度的工频磁场（ 正弦） 作不同时间辐照处理后的细胞，提取其胞浆蛋白质，定量后，以Ｗｅｓｔｅｒｎ ｂｌｏｔ 方法测定并比较细胞胞浆蛋白质酪氨酸磷酸化的程度。结果：０－４ ｍＴ 和０－８ ｍ Ｔ 两个强度的工频磁场均可影响胞浆蛋白质酪氨酸磷酸化。其中，两个强度都能影响分子量为３８ 、９７ ．４（ ×１０３） 蛋白质的酪氨酸磷酸化。此外，０ ．４ ｍＴ 强度还可使６１－７ 、１０５ 及１１２（ ×１０３） 的蛋白质酪氨酸磷酸化发生改变，而０－８ ｍＴ 强度却使７９ 、１５０（ ×１０３） 的蛋白质酪氨酸磷酸化发生变化。并且上述蛋白质酪氨酸磷酸化的变化均存在着时间相关性。结论：提示工频磁场可以使细胞内一些胞浆蛋白质酪氨酸磷酸化产生时间相关性的变化
OBJECTIVE: To study the effect of 50 Hz power frequency magnetic field on cytosolic protein tyrosine phosphorylation in order to investigate the mechanism of cell-mediated signal transduction induced by power frequency magnetic field. Methods: The cytoplasmic proteins were extracted from the irradiated magnetic field (sinusoidal) of two different intensities for different time. After quantification, the protein cytoplasmic protein tyrosine phosphates The degree of change. Results: Both the 0-4 mT and 0-8 mT power frequency magnetic fields could affect cytoplasmic protein tyrosine phosphorylation. Among them, both intensities can affect the molecular weight of 38, 97. 4 (× 103) tyrosine phosphorylation of protein. In addition, 0. The 4 mT intensity also changed protein tyrosine phosphorylation of 61-7, 105 and 112 (× 103), whereas the intensity of 0-8 mT caused 79, 150 (× 103) protein tyrosine phosphorylation Variety. And the above-mentioned changes in protein tyrosine phosphorylation are time-dependent. Conclusion: It is suggested that the frequency-frequency magnetic field can change the time-dependent phosphorylation of some cytoplasmic protein tyrosine