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目的:研究麒麟丸对雷公藤多苷所致少弱精子症模型雄性大鼠的生殖功能保护作用。方法:取28只雄性SD大鼠,随机分成正常对照组、模型组、麒麟丸低、高剂量组,除正常对照组外,其他各组灌服雷公藤多苷40 mg/(kg·d),同时麒麟丸给药组同日给予不同剂量麒麟丸保护,低剂量组给予麒麟丸1.62 g/(kg·d),高剂量组给予麒麟丸3.24 g/(kg·d)。连续给药30 d后,处死大鼠,称重大鼠睾丸,计算睾丸脏器指数,检测附睾精子浓度、活动率,检测生殖激素水平,评估抗氧化指标,HE切片观察大鼠睾丸组织形态学改变。结果:实验结束后,麒麟丸低、高剂量组精子浓度分别为(14.57±3.95)×10~6/ml、(39.71±11.31)×10~6/ml,精子活力为(3.71±1.11)%、(4.29±1.80)%皆较模型组(4.71±1.25)×10~6/ml、(0.57±0.53)%有明显提高(P<0.05);与模型组FSH(1.71±0.52)mIU/ml比较,麒麟丸低、高剂量组FSH下降,分别为(1.49±0.62)mIU/ml、(1.12±0.83)mIU/ml;fT水平较模型组升高(27.27±3.63)nmol/L、(32.80±2.51)nmol/L vs(22.81±2.75)nmol/L,SHBG水平升高(94.83±11.17)nmol/L、(88.05±9.21)nmol/L vs(56.74±8.29)nmol/L,差异皆有显著性(P<0.05),但T水平无明显变化(P>0.05)。与模型组比较,麒麟丸低、高剂量组SOD水平升高(277.14±15.84)U/ml、(299.60±20.83)U/ml vs(250.04±31.06)U/ml;ROS水平下降(397.61±62.71)IU/ml、(376.84±67.14)IU/ml vs(552.20±58.07)IU/ml(P<0.05)。与模型组比较,低、高剂量组HE染色显示麒麟丸能增加少弱精子症大鼠睾丸生精小管内各级生精细胞层次和数量。结论:麒麟丸能明显提高少弱精子症大鼠精子质量,改善生殖激素水平,减轻氧化应激损伤,改善大鼠睾丸组织形态,对生殖系统有较好的保护作用。
Objective: To study the protective effect of Kirin Pills on the reproductive function of male rats with oligozoospermia induced by tripterygium glycosides. Methods: Totally 28 male Sprague-Dawley rats were randomly divided into normal control group, model group and uniconazole low and high dose groups, except the normal control group, the other groups were given Tripterygium glycosides 40 mg / (kg · d) , Meanwhile, Kirin Pills group was given the same dose of Kirin Pills for protection on the same day. The Kirin Pills was 1.62 g / (kg · d) in the low dose group and 3.24 g / (kg · d) in the high dose group. After 30 days of continuous administration, the rats were sacrificed and the testes of rats were weighed. The testis organ index was calculated. The sperm concentration and motility of epididymis were measured. The levels of reproductive hormones were measured. Antioxidant indexes were evaluated. HE staining was used to observe the changes of testicular histomorphology . Results: At the end of the experiment, the sperm concentration of Kirin Pills group was (14.57 ± 3.95) × 10 ~ 6 / ml and (39.71 ± 11.31) × 10 ~ 6 / ml respectively and the sperm motility was (3.71 ± 1.11) (4.29 ± 1.80)% were significantly higher than those in model group (4.71 ± 1.25) × 10 ~ 6 / ml and (0.57 ± 0.53)%, respectively Compared with model group, the FSH of Kirin pill low and high dose group decreased (1.49 ± 0.62) mIU / ml and (1.12 ± 0.83) mIU / ml, respectively ± 2.51) nmol / L vs 22.81 ± 2.75 nmol / L, respectively. The levels of SHBG increased significantly (94.83 ± 11.17 nmol / L, 88.05 ± 9.21 nmol / L vs 56.74 ± 8.29 nmol / L, (P <0.05), but the level of T had no significant change (P> 0.05). Compared with the model group, the levels of SOD in Kirin Pills group were significantly higher (277.14 ± 15.84) U / ml, (299.60 ± 20.83) U / ml vs (250.04 ± 31.06) U / ml and ROS levels were lower (397.61 ± 62.71 ) IU / ml, (376.84 ± 67.14) IU / ml vs (552.20 ± 58.07) IU / ml (P <0.05). Compared with the model group, HE staining showed that Kirin Pills could increase the number and levels of spermatogenic cells in the testicular seminiferous tubule of rats with oligo-asthenospermia. Conclusion: Kirin Pills can significantly improve the sperm quality of oligoasthenospermic rats, improve the level of reproductive hormones, reduce the oxidative stress injury, improve the morphology of rat testis and have a good protective effect on the reproductive system.