论文部分内容阅读
Semicarbazide-Sensitive Amine Oxidase(SSAO)是一类在人体内广泛分布但生理功能尚不清楚的酶。本研究中我们首次在动物模型上发现了SSAO活性对大鼠血糖的影响。通过使用SSAO的高选择性抑制剂MDL-72974A以及它的内源性底物甲胺等对动物进行处理,研究血糖与SSAO活性的关系。SSAO活性用同位素标记法以14C-苯甲胺为底物进行测定,血糖则采用己糖激酶法测定。此外我们还测定了尿甲醛排泄以考察SSAO催化脱氨反应是否发生。实验结果显示,抑制SSAO活性可以导致动物血糖升高,并且SSAO底物甲胺可以降低糖尿病鼠血糖。这些结果表明SSAO可能在血糖调节方面有某种作用,进一步研究将对发展糖尿病治疗策略有重要意义。
Semicarbazide-Sensitive Amine Oxidase (SSAO) is a class of enzymes that are widely distributed in the body but whose physiological function is not known. For the first time in our study, we found the effect of SSAO activity on blood glucose in an animal model. The relationship between blood glucose and SSAO activity was studied by treating the animals with the high selective inhibitor MDL-72974A of SSAO and its endogenous substrate methylamine. SSAO activity was measured by isotope labeling with 14C-benzylamine as a substrate, and blood glucose was measured by the hexokinase method. In addition, we measured urinary formaldehyde excretion to investigate whether SSAO catalytic deamination occurred. Experimental results show that inhibition of SSAO activity can lead to elevated blood glucose in animals, and SSAO substrate methylamine can reduce blood glucose in diabetic rats. These results indicate that SSAO may have some role in the regulation of blood glucose, and further research will be of great importance to the development of diabetes treatment strategies.