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目的探讨依达拉奉对脑缺血再灌注大鼠脑组织和血清血管细胞黏附分子1(VCAM-1)和细胞间黏附分子1(ICAM-1)水平的影响。方法将30只雄性SD小鼠随机分为假手术组、缺血再灌注组和依达拉奉组,每组10只。建立大鼠大脑中动脉缺血再灌注模型,24h后采集脑组织和血清,分别采用免疫组织化学、ELISA法检测VCAM-1和ICAM-1水平。结果与假手术组比较,缺血再灌注组大鼠脑组织和血清ICAM-1、VCAM-1明显升高(P<0.05);与缺血再灌注组比较,依达拉奉组脑组织ICAM-1、VCAM-1[(0.14±0.02)μg/L vs(0.19±0.04)μg/L、(0.12±0.02)μg/L vs(0.17±0.03)μg/L,P<0.05]和血清ICAM-1、VCAM-1[(1.03±0.29)μg/L vs(1.29±0.44)μg/L、(170.79±43.42)μg/L vs(261.85±73.05)μg/L,P<0.05]明显降低。结论依达拉奉能改善脑缺血再灌注大鼠的症状,其作用机制之一是抑制ICAM-1和VCAM-1的生成。
Objective To investigate the effect of edaravone on the expression of vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in brain tissue and serum in rats with cerebral ischemia-reperfusion injury. Methods Thirty male SD mice were randomly divided into sham operation group, ischemia reperfusion group and edaravone group. The rat middle cerebral artery occlusion (MCAO) model was established and the brain tissue and serum were collected 24h later. The levels of VCAM-1 and ICAM-1 were detected by immunohistochemistry and ELISA respectively. Results Compared with the sham operation group, the levels of ICAM-1 and VCAM-1 in the brain tissue and serum in the ischemia-reperfusion group were significantly increased (P <0.05). Compared with the ischemia-reperfusion group, the ICAM- 1, (0.14 ± 0.02) μg / L vs (0.19 ± 0.04) μg / L, (0.12 ± 0.02) μg / L vs (0.17 ± 0.03) μg / L, P < -1, VCAM-1 [(1.03 ± 0.29) μg / L vs (1.29 ± 0.44) μg / L, (170.79 ± 43.42) μg / L vs (261.85 ± 73.05) μg / L, P <0.05]. Conclusion Edaravone can ameliorate the symptoms of rats with cerebral ischemia and reperfusion. One of the mechanisms is that it inhibits the production of ICAM-1 and VCAM-1.