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目的建立测定天柏金黄散中白芷有效成分欧前胡素含量的方法。方法采用高效液相色谱法,色谱柱为ThermoODS Hypersil(250×4.6mm 5μm),流动相为甲醇-水(60 40),检测波长为300nm,流速为1.0ml.min-1。结果欧前胡素的检测浓度在4.03-400.3μg.ml-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为100.06%,RSD=0.79%(n=9)。结论本方法简单、准确、重复性好,可用于天柏金黄散的质量控制。
Objective To establish a method for determining the content of Imperatorin in Angelica dahuricae. Methods The column was ThermoODS Hypersil (250 × 4.6mm 5μm). The mobile phase was methanol-water (60:40). The detection wavelength was 300nm and the flow rate was 1.0ml.min-1. Results The concentration of Imperatorin showed a good linear relationship with the peak area integral in the range of 4.03-400.3μg.ml-1 (r = 0.9999). The average recovery was 100.06%, RSD was 0.79% (n = 9) . Conclusion The method is simple, accurate and reproducible. It can be used for the quality control of Tianbai golden yellow powder.