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目的探讨粮食污染造成的氟中毒对仔鼠学习记忆影响及其作用机制。方法SD大鼠随机分为对照组、高氟组。高氟组以地氟病区燃煤烘烤的玉米为主要饲料,复制氟中毒动物模型,饲养6个月后,取雌雄合笼后生产的30 d龄仔鼠,采用Morris水迷宫方法检测仔鼠行为学变化,应用生物化学方法测定脑组织胆碱酯酶活性。结果高氟组仔鼠逃避潜伏期时间为(9.242±5.852)s,与对照组仔鼠(4.948±2.698)s比较,明显延长(P<0.01);高氟组仔鼠第7d穿过平台次数和逗留平台象限时间分别为(3.040±1.968)次,(23.070±5.427)s,与对照组仔鼠比较,均明显降低(P<0.05,P<0.01);高氟组仔鼠脑组织中胆碱酯酶活性明显低于对照组(P<0.01);胆碱酯酶活性与逃避潜伏期呈负相关,与第7 d穿过平台次数和逗留平台象限时间呈正相关。结论过量氟可引起仔鼠空间学习记忆能力降低,其机制可能与脑组织胆碱酯酶活性下降有关。
Objective To investigate the effects of fluoride poisoning on learning and memory in offspring rats induced by food contamination and its mechanism. Methods SD rats were randomly divided into control group and high fluoride group. In the high-fluoride group, the corn fed the coal-fired ground burns in the endemic fluorosis area was used as the main feed to reproduce the fluoride poisoning animal model. After feeding for 6 months, the 30-day-old offspring were housed in male and female cages. Morris water maze test Changes in rat behavior, the use of biochemical methods for determination of brain tissue cholinesterase activity. Results The escape latency of the offspring in high fluoride group was (9.242 ± 5.852) s, which was significantly longer than that in the control group (4.948 ± 2.698) s (P <0.01) (3.040 ± 1.968) and (23.070 ± 5.427) s, respectively, which were significantly lower than those in the control group (P <0.05, P <0.01). The level of choline The esterase activity was significantly lower than that of the control group (P <0.01). The cholinesterase activity was negatively correlated with the escape latency and positively correlated with the number of platforms passing through the platform and the quadrant time of the platform. Conclusions Excessive fluoride can decrease the spatial learning and memory abilities in offspring rats. The mechanism may be related to the decrease of cholinesterase activity in brain tissue.