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在含有２ｍｇ／ＬＩＡＡ和３ｍｇ／ＬＢＡＰ的改良Ｎ６培养基上，香雪兰（ＦｒｅｓｉａｒｅｆｒａｃｔａＫｌａｔ）花序外植体经直接体细胞胚胎发生途径再生出新的植株。在这一形态发生过程中，一个引人注意的现象是，所有的体细胞胚都出现在花序轴切段的原形态学下端（称为胚发生端，ＥＥ），而在形态学上端（称为非胚胎发生端，ＮＥＥ）无体细胞胚形成。这一形态发生的极性与地心引力的方向和外植体在培养基上的放置位置无关。在培养的早期，仅于胚发生端观察到了起始细胞的分裂。ＳＤＳ聚丙烯酰胺凝胶电泳结果表明，在培养了１ｄ的外植体的胚发生端出现了两个特殊的多肽成分，而在非胚发生端则未检测到这两种多肽。高效液相色谱分析表明，培养前外植体切段两端的内源激素（ＩＡＡ）的含量无显著差别。但经过一段时间的培养，胚发生端的ＩＡＡ含量明显高于非胚发生端的ＩＡＡ含量，表明内源激素在体细胞胚胎发生的诱导过程中起着关键的作用。在香雪兰体细胞胚胎发生诱导的过程中，由于花序轴切段两端在分子水平和生理水平上均存在差异，使这一系统有可能成为研究体细胞胚胎发生机理的有用实验材料。
Fresia refractaKlat inflorescence explants regenerated new plants via direct somatic embryogenesis on modified N6 medium containing 2 mg / L IAA and 3 mg / LBAP. An interesting phenomenon in this morphogenesis is that all the somatic embryos occur at the lower end of the original morphology of the axon of the inflorescence (called the embryogenesis end, EE), while at the morphological upper end Non-embryogenic terminal, NEE) somatic embryogenesis. The polarity of this morphogenesis has nothing to do with the direction of gravity and the placement of explants on the medium. In the early stages of culture, initiation cell division was observed only at the embryogenic site. SDS-polyacrylamide gel electrophoresis results showed that there were two special polypeptide components in the embryogenesis end of the explants cultured for 1 day, while the two polypeptides were not detected in the non-embryogenic end. High performance liquid chromatography analysis showed that there was no significant difference in endogenous hormones (IAA) content at both ends of explant before culture. However, after a period of incubation, the content of IAA at embryogenesis was significantly higher than that at non-embryogenesis, indicating that endogenous hormones played a key role in the induction of somatic embryogenesis. During the induction of Solanum solanum embryogenesis, there is a possibility that this system may become a useful experimental material for studying the mechanism of somatic embryogenesis due to the difference in molecular level and physiological level at both ends of the axon.