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应用间接免疫荧光抗体法可在部分硬皮病CREST综合征患者血清中发现与染色体着丝粒部分有特异反应的抗体,称抗着丝粒抗体(ACA)。着丝粒抗原是一类蛋白质,含CENP-A(17kd)、CENP-B(80kd)及CENP-C(140kd)3种组分,只有具有功能的着丝粒才与ACA发生反应,故ACA对了解着丝粒的活性功能、形态及数目有重要意义。研究双着丝粒染色体、罗伯逊易位等染色体异常时,多年来主要采用包括C显带在内的不同显带技术,但这些方法均不能显示着丝粒的活性功能,由于ACA只特异结合予着丝粒蛋白上,从而解决了这一问题。在染色体异常中,双着丝粒染色体及各种易位的染色体很常见,故对培养的外周血淋巴细胞建立ACA间接免疫荧光方法可能有更重要的临床应用意义。我们参考文献报道,结合本室工作,改进了适用于培养的外周血淋巴细胞ACA方法,现报告如下。
Indirect immunofluorescence antibody method can be found in the serum of some patients with scleroderma CREST syndrome and chromosome centromere part of the specific reaction of antibodies, called anti-centromere antibody (ACA). Centromere antigens are a class of proteins that contain CENP-A (17kd), CENP-B (80kd) and CENP-C (140kd). Only functional centromere reacts with ACA, To understand the activity of centromere function, shape and number of great significance. When studying chromosomal abnormalities such as dicentric chromosomes and Robertsonian translocations, different banding techniques including C-banding have been used for many years. However, these methods do not display the activity of centromere. Since ACA only specifically binds Centromere protein, thus solving this problem. In chromosomal abnormalities, dicentric chromosomes and a variety of translocations of chromosomes are common, so the establishment of indirect immunofluorescence assay of cultured peripheral blood lymphocytes may have more important clinical significance. Our reference reports, combined with the work room, improved for the training of peripheral blood lymphocytes ACA method, are as follows.