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目的 观察白念珠菌(白念菌)在肠腔内增殖与局部粘膜免疫反应的关系。方法 采用无特殊病原菌动物经口喂入白念菌,在不同时相处死后,观察肠内白念菌总数及肠粘膜表面白念菌粘附数量;取肠系膜淋巴结做白念菌选择培养,观察移位体内发生率;采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况;流式细胞计数潘伊尔氏结细胞表面IgA(SmIgA)阳性率;免疫组化染色后计数固有层中IgA浆细胞数量变化;ELISA法测定肠粘液中特异抗白念菌IgA含量。结果 肠内白念菌总量及粘膜表面粘附数量随给菌后时间延长而明显下降;移位主要发生于给菌后早期;给菌后固有层淋巴细胞增殖活跃,分泌IgA浆细胞数量明显增加;粘液层中特异抗白念菌IgA含量在给菌后上升明显。结论 带菌动物首次白念菌肠内给入后以固有层中淋巴细胞反应为主,增殖及分化均增加;白念菌总量和粘附数量减少与局部粘膜免疫反应及特异IgA抗体含量上升的关系十分密切。
Objective To observe the relationship between Candida albicans (Candida albicans) proliferation in the intestine and local mucosal immune response. Methods Animals without pathogenic bacteria were orally fed with Candida albicans and observed the total number of Candida albicans and the number of Candida albicans adhered on the intestinal mucosa after sacrificing at different time. Mesenteric lymph nodes were selected for culture of Candida albicans. The rate of local IgA (SmIgA) staining was detected by flow cytometry. The number of IgA plasma cells in the lamina propria was measured by immunohistochemistry The quantity of IgA was detected by ELISA in intestinal mucus. Results The total amount of intestinal Candida albicans and the number of mucosal surface adhesion significantly decreased with the prolongation of time after transdermal delivery. The translocation mainly occurred in the early stage of post-transdermal delivery. The number of IgA-secreting cells in the lamina propria was increased significantly Increase; mucus layer of anti-albicans IgA content increased significantly after giving the bacteria. CONCLUSION: The first reaction of white carnivore was carried out by intestinal lymphocyte reaction in intestinal lamina propria, and the proliferation and differentiation were all increased. The total amount and adhesion of Candida albicans decreased with the increase of local mucosal immune response and specific IgA antibody The relationship is very close.