论文部分内容阅读
目的探讨阿霉素(ADM)肾病大鼠Th17相关的IL-17和Treg相关的IL-10表达水平及意义。方法采用一次性尾静脉注射阿霉素的方法制备大鼠ADM模型,对照组注射等量的生理盐水,ADM组20只大鼠在造模后尿蛋白均达到50mg/24h以上,表明造模成功,采用流式细胞术检测外周血中Th17细胞和Treg细胞数量及比例;ELISA法检测各组血清和肾组织中Th17相关的IL-17和Treg相关IL-10水平。结果 ADM模型组大鼠在第14天出现蛋白尿,随着时间的推移,蛋白尿的量持续升高,在第56天达到峰值,各时间点(14,28,42,56d)ADM组大鼠的蛋白尿水平明显高于同时间的对照组(P<0.001);ADM组和对照组Th17细胞百分率分别为(3.13±0.09)%、(0.88±0.05)%,ADM组较对照组明显升高(P<0.001);ADM组和对照组Treg细胞百分率分别为(1.94±0.15)%、(5.02±0.15)%,ADM组明显低于对照组(P<0.001);ADM组Th17/Treg细胞比例较对照组高(P<0.001),ADM组大鼠(14,28,42,56d)的血清及肾组织IL-17表达水平明显高于对照组(P<0.001);IL-10表达水平明显低于对照组(P<0.001)。结论 Th17/Treg为主的免疫失衡在ADM发病中起重要作用,可通过抑制或中和内源性IL-17的表达和提升Treg的表达纠正失衡的Th17/Treg,此方法可能成为儿童原发性肾病(PNS)治疗的新途径之一。
Objective To investigate the expression and significance of Th17-associated IL-17 and Treg-related IL-10 in rats with adriamycin (ADM) nephropathy. Methods Adriamycin was injected into the tail vein to prepare ADM model. The control group was given the same amount of saline. The urine protein of 20 rats in the ADM group reached 50mg / 24h or more after modeling, indicating that the model was successful The number and proportion of Th17 cells and Treg cells in peripheral blood were detected by flow cytometry. The levels of Th17-associated IL-17 and Treg-related IL-10 in serum and kidney tissue of each group were detected by ELISA. RESULTS: Proteinuria occurred on the 14th day in ADM model rats. The proteinuria volume continued to increase with the passage of time and reached the peak on the 56th day. At each time point (14, 28, 42, 56 days), the ADM group was larger (P <0.001). The percentage of Th17 cells in ADM group and control group were (3.13 ± 0.09)% and (0.88 ± 0.05)%, respectively. The levels of Th17 cells in ADM group and control group were significantly higher than those in control group (P <0.001). The percentage of Treg cells in ADM group and control group were (1.94 ± 0.15)% and (5.02 ± 0.15)% respectively, which were significantly lower in ADM group than those in control group (P <0.001). The levels of IL-17 in serum and kidney of ADM group (14,28,42,56 d) were significantly higher than those in control group (P <0.001) Significantly lower than the control group (P <0.001). Conclusion Th17 / Treg-based immune imbalance plays an important role in the pathogenesis of ADM. It can correct imbalanced Th17 / Treg by inhibiting or neutralizing the expression of endogenous IL-17 and increasing the expression of Treg, One of the new ways of treating nephropathy (PNS).