平菇用菇体组织分离和菇木分离制母种,菇体、菇木表面一般都先用0.1％升汞或75％酒精进行消毒。其实菇体、菇木表面消毒只能杀死部分杂菌,对于平菇这样的大型子实体,只要严格掌握无菌操作技术,无须对分离材料进行表面消毒,所接的母种杂菌感染率很低,成功率几乎达100％。现将操作技术介绍如下: Pleurotus ostreatus Mushroom tissue separation and separation of mushrooms mother species, mushroom body, mushroom wood surface are generally first 0.1% mercuric chloride or 75% alcohol for disinfection. In fact, mushrooms, mushrooms, wood surface disinfection can only kill part of the bacteria, mushrooms such large-scale fruiting bodies, as long as a strict grasp of aseptic technique, without separation of materials for surface disinfection, access to the mother of bacteria infection rate Very low, the success rate is almost 100%. Now the operation of the technology are as follows: