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聚合酶链式反应（PCR）由Mullis于1985年发明。作为一种新颖的基因诊断技术已在遗传病及感染性疾病的诊断中广泛应用。PCR技术被誉为对医学、生物学技术的一场革命和建立了新的里程碑，为此Mullis荣获了1993年的诺贝尔奖。该技术基本原理同DNA的天然复制。实验时在耐热DNA聚合
Polymerase chain reaction (PCR) was invented by Mullis in 1985. As a novel genetic diagnostic technology has been widely used in the diagnosis of genetic diseases and infectious diseases. PCR technology was hailed as a revolution in medical and biological technology and established a new milestone, for which Mullis won the 1993 Nobel Prize. The basic principle of this technique is the same as the natural replication of DNA. Experiments in thermostable DNA polymerization