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目的建立RP-HPLC测定血清中文拉法辛(VL)及其活性代谢产物O-去甲文拉法辛(ODV)的浓度。方法血清样品碱化后采用乙醚萃取,其有机相再用盐酸反提取。采用VP-ODS柱,以甲醇-水-正丁胺-冰醋酸(54∶60∶1.5∶1.2)为流动相,UV检测波长为229nm。结果血清文拉法辛及O-去甲文拉法辛线性范围均为31.25～500μg·L-1,方法回收率分别为92.3%～125.7%及109.9%～121.8%,日内RSD分别为5.52%～7.70%及1.76%～9.46%,日间RSD分别为4.59%～7.70%及1.96%～9.59%。结论本法准确可靠,重现性好,适用于文拉法辛及O-去甲文拉法辛人体药动学研究中血药浓度的测定。
OBJECTIVE To establish a RP-HPLC method for the determination of serum concentration of lavazine (VL) and its active metabolite O-desvenlafaxine (ODV). Methods Serum samples were extracted with ether after basification and the organic phase was extracted with hydrochloric acid again. The mobile phase was VP-ODS column with methanol-water-n-butylamine-glacial acetic acid (54:60:1.5:1.2). The UV detection wavelength was 229nm. Results The linear ranges of serum venlafaxine and o-desvenlafaxine were both 31.25-500 μg · L-1. The recovery rates were 92.3% -125.7% and 109.9% -121.8%, respectively. The intra-day RSD were 5.52% ~ 7.70% and 1.76% ~ 9.46% respectively. The daytime RSD were 4.59% ~ 7.70% and 1.96% ~ 9.59%, respectively. Conclusion This method is accurate and reproducible, and it is suitable for the determination of plasma concentration of venlafaxine and o-desvenlafaxine in human pharmacokinetic studies.