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细胞免疫能增强巨噬细胞的活性。小鼠接种照射的曼氏血吸虫尾蚴或小剂量持续感染都会对曼氏血吸虫产生部分抵抗力或保护作用。本文作者用50,000rad γ射线照射昆蚴,使C57BL/6J小鼠尾部皮肤与其接触感染而产生免疫。然后用纯种曼氏血吸虫成虫制备可溶性抗原SWAP,把SWAP溶于PBS中。接着用SWAP溶液对接种后的小鼠在左右后足进行皮试并用PBS对照,以观察小鼠体内发生的免疫反应。同时又用SWAP及活的或裂解的血吸虫作为抗原,制备不同的体外培养剂,将接种后小鼠的脾细胞在体外培养,以观察脾细胞对这些抗原的反应,并将反应产物成分提取加以分析。
Cellular immunity enhances the activity of macrophages. Inoculation of mice infected with Schistosoma mansoni cercariae or small doses of persistent infection will have some resistance or protection against Schistosoma mansoni. The author of this study used 50,000 rad γ-rays to irradiate the larvae of Echinococcus granulosus to immunize the tail skin of C57BL / 6J mice in contact with their infection. Soluble antigen SWAP was then prepared from the pure adult Schistosoma mansoni and the SWAP was dissolved in PBS. Subsequent mice were inoculated with SWAP solution in a skin test on the left and right hind paws and compared with PBS to observe the immune response that occurred in the mice. At the same time, SWAP and live or lysed Schistosoma japonicum were used as antigens to prepare different in vitro culture medium. The spleen cells of mice after inoculation were cultured in vitro to observe the response of spleen cells to these antigens and extract the reaction product components analysis.