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目的研究左氧氟沙星联合清解剂对铜绿假单胞菌抑菌能力及防耐药突变浓度(mutant preventionconcentration,MPC)的影响。方法采用微量肉汤二倍稀释法测定左氧氟沙星、清解剂单独使用及两药联用的最低抑菌浓度(minimum inhibitory concentration,MIC)和防耐药变异浓度,并计算联合用药后的突变选择窗(mutant selectionwindows,MSW)。结果左氧氟沙星、清解剂单用对铜绿假单孢菌的MIC分别为1.0μg/m L、5.0 mg/m L,MPC分别为5.12μg/m L、200 mg/m L。左氧氟沙星与1MIC的清解剂联用后可使左氧氟沙星对铜绿假单胞菌的MIC降到0.5μg/m L,MPC下降至3.2μg/m L。结论左氧氟沙星与清解剂联用,具有相加作用,可以明显降低各自单独用药对铜绿假单胞菌的MIC及MPC。为优化临床用药方案、防止细菌耐药性突变株的产生提供一定的理论依据。
Objective To study the effect of levofloxacin combined with clearing agents on the antibacterial activity and the mutant prevention concentration (MPC) of Pseudomonas aeruginosa. Methods The minimum inhibitory concentration (MIC) and concentration of anti-drug resistance of levofloxacin, the agent of clearing agent and the combination of the two drugs were determined by the method of double broth microdilution. The mutation selection window (mutant selectionwindows, MSW). Results MICs of levofloxacin and decontamination agents against Pseudomonas aeruginosa alone were 1.0 μg / m L and 5.0 mg / m L, respectively. The MPC values were 5.12 μg / m L and 200 mg / m L, respectively. Levofloxacin and 1 MIC combined with clenbuterol levofloxacin Pseudomonas aeruginosa MIC decreased to 0.5μg / m L, MPC decreased to 3.2μg / m L. Conclusion Levofloxacin combined with clearing agent, with the additive effect, can significantly reduce their respective drugs on the Pseudomonas aeruginosa MIC and MPC. In order to optimize the clinical medication program to prevent the emergence of bacterial resistance mutations provide a theoretical basis.