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目的探讨血管内皮生长因子(VEGF)和Cleaved Caspase-3在宫颈鳞癌组织中的表达及临床意义。方法应用免疫组织化学法、荧光实时定量聚合酶链反应(PCR)和Western blot法分别检测在46例慢性宫颈炎和52例宫颈鳞癌组织中VEGF和Cleaved Caspase-3的表达情况。结果VEGF和Cleaved Caspase-3在不同组织中的相对表达量均不同,慢性宫颈炎组的VEGF基因和蛋白表达量明显低于宫颈鳞癌组(P<0.01)。Cleaved Caspase-3基因和蛋白表达呈相反趋势。VEGF和Cleaved Caspase-3表达均与淋巴结转移、国际妇产科联盟(FIGO)分期有关,差异均有统计学意义(P<0.05)。结论在宫颈鳞癌组织中,VEGF过表达,但Cleaved Caspase-3呈低表达,两者均参与了宫颈鳞癌的发生发展,其联合检测有助于判断患者的预后。
Objective To investigate the expression and clinical significance of vascular endothelial growth factor (VEGF) and Cleaved Caspase-3 in cervical squamous cell carcinoma. Methods The expression of VEGF and Cleaved Caspase-3 in 46 cases of chronic cervicitis and 52 cases of cervical squamous cell carcinoma were detected by immunohistochemistry, real-time quantitative polymerase chain reaction (PCR) and Western blot respectively. Results The relative expression levels of VEGF and Cleaved Caspase-3 in different tissues were different. The expression of VEGF gene and protein in chronic cervicitis group was significantly lower than that in cervical squamous cell carcinoma group (P <0.01). Cleaved Caspase-3 gene and protein expression showed the opposite trend. The expressions of VEGF and Cleaved Caspase-3 were correlated with lymph node metastasis and FIGO staging (P <0.05). Conclusion The expression of VEGF is overexpressed in cervical squamous cell carcinoma, but the expression of Cleaved Caspase-3 is low, both of which are involved in the occurrence and development of cervical squamous cell carcinoma. The combined detection of VEGF and VEGF may help to judge the prognosis of patients.