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目的探讨黄芪对大鼠脑出血后神经元线粒体的保护作用。方法用Ⅶ型胶原酶脑内注入法制作大鼠脑出血模型,利用Narrow-alley Test检测各组大鼠的神经行为学指标;用透射电镜观察各组大鼠脑出血灶周围神经元线粒体的超微结构并对其进行体视学分析;用免疫组织化学方法检测Caspase-3蛋白表达。结果神经行为学检测,模型组大鼠的不对称分值明显升高,治疗组大鼠不对称分值较模型组明显下降;神经元线粒体体视学分析,与模型组相比,治疗组线粒体的体密度、数密度、比表面积和比膜面积均较模型组增大,差异有显著性(P<0.05);与模型组比较,治疗组Caspase-3的阳性表达明显降低(P<0.01)。结论黄芪能够减轻脑出血后神经元线粒体的损伤,抑制神经元凋亡,促进神经功能恢复。
Objective To investigate the protective effects of Astragalus mongholicus on neuronal mitochondria after intracerebral hemorrhage in rats. Methods The intracerebral hemorrhage model was made by injection of collagenase type Ⅶ in rats. The neurobehavioral indexes of rats in each group were detected by Narrow-alley Test. The mitochondrial ultrastructural changes of neurons in cerebral hemorrhage were observed by transmission electron microscope Microstructure and stereological analysis; Caspase-3 protein expression was detected by immunohistochemistry. Results The neurobehavioral tests showed that the asymmetry score of the model group was significantly higher than that of the untreated group and that of the untreated group was significantly lower than that of the model group. Compared with the model group, the mitochondria (P <0.05). Compared with the model group, the positive expression of Caspase-3 in the treatment group was significantly lower than that in the model group (P <0.01), the density, specific surface area and specific membrane area increased significantly . Conclusion Astragalus can alleviate the neuronal mitochondrial damage after intracerebral hemorrhage, inhibit neuronal apoptosis and promote neurological recovery.