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肝癌发生与组织中微量元素水平和一些酶的活性相关。为研究肝癌组织内金属蛋白的分布特征并寻找有意义的微量元素存在形式加以深入研究,用等电聚焦(IEF)分离肝细胞癌以及癌旁组织细胞胞质溶胶内的蛋白质,用同步辐射X荧光分析蛋白条带内的金属含量。初步研究表明:在肝细胞癌和癌旁组织的细胞胞质溶胶中所检出的含铁、铜、锌蛋白基本是一致的,一般癌旁组织蛋白质的铁、铜、锌含量高于癌组织,癌旁组织中铁含量高主要体现在pI为6.5、7.7、8.0及小于3.5的含铁条带内,而癌组织中pI为4.0、7.0的条带内铁含量略高于癌旁组织,癌旁组织的pl为3.2、4.9、5.5、5.9和6.5的条带铜含量明显高于癌组织,锌含量的升高则主要体现在pI为5.5和6.5的含锌条带内,这些分布特征的普遍性及生物学意义有待进一步研究。
The occurrence of HCC correlates with the level of trace elements in tissues and the activity of some enzymes. In order to study the distribution characteristics of metalloprotein in hepatocellular carcinoma and to find out the existence forms of trace elements in depth, the IEF was used to separate the proteins in the cytosol of hepatocellular carcinoma and paracancerous tissues. The synaptic radiation X Fluorescence analysis of metal content in protein bands. Preliminary studies have shown that: iron, copper, zinc protein detected in the cytosol of hepatocellular carcinoma and paracancerous tissue is basically the same, the content of iron, copper, zinc of the paracancer tissue protein is generally higher than that of the cancer tissue , While the high content of iron in paracancerous tissues is mainly reflected in the iron-containing bands with pI of 6.5, 7.7, 8.0 and less than 3.5, while the intra-abdominal iron content of pI of cancer tissues of 4.0 and 7.0 is slightly higher than that of adjacent tissues In the adjacent tissues, the copper content in the strips with 3.2, 4.9, 5.5, 5.9 and 6.5 was significantly higher than that in the cancerous tissues. The increase in zinc content was mainly found in zinc-containing strips with pI of 5.5 and 6.5. The universality and biological significance need further study.