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基于电子克隆获得的大豆GmAHRI基因cDNA序列编码区设计特异引物,以高异亮氨酸大豆品种“和龙早熟豆”总RNA为模板,通过RT-PCR获得了约1 764 bp的cDNA片段,T/A克隆后进行序列测定。测序结果显示:GmAHRI基因家族有2个成员,分别命名为GmAHRI1(FJ594399.1)和GmAHRI2(JN034043)。GmAHRI1和GmAHRI2的编码区长度均为1 764 bp,编码587个氨基酸,由10个外显子和9个内含子构成,分别定位于Gm12和Gm13染色体上。2个基因在氨基酸水平上的相似性达到了96.71%,所编码的蛋白质在二级结构上有所差异,三级结构相同。
Based on the cDNA sequence coding region of soybean GmAHRI gene obtained by electronic cloning, a specific primer was designed and a cDNA fragment of about 1 764 bp was obtained by RT-PCR using isoleucine soybean variety “Heilongjiang” as template. T / A cloning sequence analysis. The sequencing results showed that the GmAHRI gene family has two members, named GmAHRI1 (FJ594399.1) and GmAHRI2 (JN034043) respectively. The coding regions of GmAHRI1 and GmAHRI2 are both 1 764 bp in length and encode 587 amino acids. They consist of 10 exons and 9 introns and are located on the Gm12 and Gm13 chromosomes respectively. The similarity of the two genes at the amino acid level reached 96.71%. The encoded proteins differed in secondary structure and the tertiary structure was the same.