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目的 研究p16基因突变与食管癌和胃癌发生的关系。方法 采用PCR、多重PCR、PCR SSCP和DNA测序等技术分析了 2 5例食管癌和 40例胃癌组织标本。结果 1.食管癌中p16基因突变频率为 16 % ,并且突变频率与肿瘤的大小、位置、分化程度和有无淋巴结转移无关 (P >0 .0 5 )。 2 .胃癌中p16基因突变频率为 7.5 % ,并且突变与肿瘤的大小、位置、分化程度和有无淋巴结转移无关 (P >0 .0 5 )。结论 1.P16基因点突变可能是食管癌和胃癌发生发展过程中的影响因素之一 ,错义突变是其失活的主要原因。 2 .P16基因点突变的检测对食管癌和胃癌早期诊断有一定帮助。
Objective To study the relationship between p16 gene mutation and esophageal cancer and gastric cancer. Methods Twenty-five cases of esophageal cancer and 40 cases of gastric cancer were analyzed by PCR, multiplex PCR, PCR SSCP and DNA sequencing. The frequency of p16 gene mutation in esophageal cancer was 16%, and the frequency of mutation was not related to tumor size, location, degree of differentiation and lymph node metastasis (P> 0.05). The frequency of p16 gene mutation in gastric cancer was 7.5%, and the mutation was not associated with tumor size, location, degree of differentiation and lymph node metastasis (P> 0.05). Mutations in P16 gene may be one of the influencing factors in the development and progression of esophageal cancer and gastric cancer, missense mutation is the main reason for its inactivation. The detection of P16 gene point mutation will be helpful to the early diagnosis of esophageal cancer and gastric cancer.