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目的 通过离体气管的不同制备方法 ,分析其各自的特点 ,选择适用于学生实验的最佳制备方法。方法 取健康豚鼠的离体气管 ,分别制备成 :气管片、气管连环和气管螺旋条 ,分别观察它们对相关生理活性物质的反应。结果 气管螺旋条和气管连环因药物引起的收缩、舒张作用明显 ,气管螺旋条作用最强 ,而气管片的反应弱。结论 气管螺旋条制备法适用于学生生理机能实验。
Objective Through different preparation methods of isolated trachea, analyze their own characteristics, select the best preparation method suitable for students’ experiments. Methods Ex vivo tracheas of healthy guinea pigs were prepared, respectively: trachea, trachea and trachea spiral strips, respectively, to observe their response to the relevant physiologically active substances. Results Tracheal spiral and tracheal chain due to drug induced contraction, diastolic obvious effect of the strongest tracheal spiral, and the tracheal piece of the reaction is weak. Conclusion Tracheal spiral strip preparation method is suitable for students’ physiological function experiment.