论文部分内容阅读
目的优化女贞叶有效成分提取工艺的研究。方法以总皂苷提取含量和总黄酮提取含量的综合评分为因变量,以提取时间,溶媒用量(乙醇体积分数),料液比,提取次数为自变量,采用Box-Behnken响应面法优选女贞叶的有效成分提取工艺,应用Design Expert.8.061软件分析最终所得实验数据,建立多元二次回归方程。结果优选出女贞叶的最佳提取工艺参数为:乙醇体积分数为89.66%,提取次数2.47次,料液比11.41∶1,提取时间57.60 min,考虑到实际可操作性及工业大生产的实用性,把提取工艺参数设定为乙醇体积分数90%,料液比11∶1,提取时间60 min,提取次数2次。最终的实测值0.98619与预测值0.97323的吻合度良好。结论以总黄酮、总皂苷的提取含量的综合评分优选出女贞叶的提取工艺,为建立女贞叶合理科学的提取工艺提供参考。
Objective To optimize the extraction of active ingredients from Ligustrum lucidum leaves. Methods The total score of total saponin extraction and total flavonoids extraction was taken as the dependent variable, and the extraction time, the amount of solvent (ethanol volume fraction), the ratio of solid to liquid and the number of extraction were used as independent variables. The Box-Behnken response surface method Effective components of the leaf extraction process, the application of Design Expert.8.061 software analysis of the final experimental data to establish multivariate quadratic regression equation. The results showed that the optimal extraction parameters of privet leaves were: ethanol volume fraction 89.66%, extraction times 2.47 times, solid-liquid ratio 11.41:1 and extraction time 57.60 min. Considering the practical operability and practicality of industrial production The extraction process parameters were set as follows: ethanol volume fraction 90%, solid-liquid ratio 11: 1, extraction time 60 min, extraction times 2 times. The good agreement between the final measured value of 0.98619 and the predicted value of 0.97323 was good. Conclusion The extraction process of Ligustrum lucidum leaves was optimized by the comprehensive score of total flavonoids and total saponins, which provided a scientific basis for the establishment of lustrous leaves extraction technology.