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采用双侧海马CA1区注射Aβ42构建阿尔茨海默症(Alzheimer′s disease,AD)动物模型,通过穿梭箱被动回避记录行为学数据;以大鼠血清和脑组织中的总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)活力及还原型谷胱甘肽(GSH)、丙二醛(MDA)和蛋白质羰基(PC)含量作为评价指标,分析黑果枸杞花青素(OPC)样品对AD模型大鼠记忆力以及体内抗氧化活性的影响。结果表明,模型组动物的记忆能力显著下降,而黑果枸杞花青素组可改善AD模型大鼠的记忆损伤;同时,发现灌胃剂量为80 mg/kg的黑果枸杞花青素组能显著提高AD大鼠血清和脑组织中T-SOD、CAT活力和GSH含量,并降低MDA和蛋白质羰基含量水平。综合上述结果可知,本研究所述黑果枸杞花青素具有良好的增强体内抗氧化活性和提高AD大鼠记忆力的作用,并具有预防AD的潜在功效。
The animal model of Alzheimer’s disease (AD) was established by injecting Aβ42 into hippocampal CA1 region of the bilateral hippocampus, and the behavioral data were passively avoided by the shuttle box. The total superoxide dismutase (T-SOD), catalase (CAT) activity and glutathione (GSH), malondialdehyde (MDA) and protein carbonyl content (PC) Effects of OPC on the Memory and Antioxidant Activity in AD Model Rats. The results showed that the memory ability of the model group decreased significantly, while the black fruit Lycium barbarum anthocyanin group could improve the memory impairment of the AD model rats. At the same time, it was found that the blackberry Lycium barbarum anthocyanin group with a dose of 80 mg / kg Significantly increase T-SOD, CAT activity and GSH content in serum and brain tissue of AD rats, and reduce the levels of MDA and protein carbonyls. Based on the above results, we know that the black fruit Lycium barbarum anthocyanins in this study have a good effect of enhancing antioxidant activity in vivo and improving the memory of AD rats, and have the potential efficacy of preventing AD.