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为查明丽江烟草漂浮苗病毒病原及有效预防烟草花叶病毒病提供依据,2006～2011年对丽江4县1区主要育苗点烟草漂浮苗进行随机抽样法取样采集16 201份烟苗样品进行病毒血清学及形态学检测鉴定。结果表明:所采集的样品中仅检测出烟草花叶病毒,6年丽江总体烟草花叶病病毒病原阳性率占3.01%,表明烟草花叶病毒是危害丽江烟草漂浮苗的重要病毒病原。整体上丽江烟苗病毒病原阳性率由2006～2008的3.87%下降为2009～201l的2.14%这主要是漂浮育苗技术推广的结果。仍出现较高的病毒病原阳性率的个别地方,需要加强育苗操作的管理。
In order to find out the pathogen of Lijiang tobacco floating virus and to effectively prevent tobacco mosaic virus disease, from 2006 to 2011, 16 201 samples of tobacco seedlings were collected by sampling randomly Serological and morphological detection and identification. The results showed that only the Mosaic virus was detected in the collected samples, and the positive rate of Lijiang overall tobacco mosaic virus was 3.01% in 6 years, indicating that tobacco mosaic virus is an important viral pathogen that endangers Lijiang tobacco floating seedlings. Overall, the positive rate of Lijiang tobacco seedlings virus pathogen decreased from 3.87% in 2006 ~ 2008 to 2.14% in 2009 ~ 201l. This is mainly the result of floating seedling technology promotion. In some places where there is still a high positive rate of virus pathogen, management of nursery operations needs to be strengthened.